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J Bacteriol, April 1998, p. 2212-2219, Vol. 180, No. 8
Department of Microbiology, University of
Iowa, Iowa City, Iowa 52242
Received 11 September 1997/Accepted 6 February 1998
The gene fgd, which codes for
F420-dependent glucose-6-phosphate dehydrogenase (FGD), was
cloned from Mycobacterium smegmatis, and its sequence was
determined and analyzed. A homolog of FGD which has a very high
similarity to the M. smegmatis FGD-derived amino acid
sequence was identified in Mycobacterium tuberculosis. FGD
showed significant homology with F420-dependent
N5,N10-methylene-tetrahydromethanopterin
reductase (MER) from methanogenic archaea and with several
hypothetical proteins from M. tuberculosis and
Archaeoglobus fulgidus, but FGD showed no
significant homology with NADP-dependent glucose-6-phosphate
dehydrogenases. Multiple alignment of FGD and MER proteins revealed
four conserved consensus sequences. Multiple alignment of FGD with the
hypothetical proteins also revealed portions of the same conserved
sequences. Moderately high levels of FGD were expressed in
Escherichia coli BL21(DE3) carrying fgd in
pBluescript.
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Molecular Analysis of the Gene Encoding
F420-Dependent Glucose-6-Phosphate Dehydrogenase from
Mycobacterium smegmatis
and
*
Corresponding author. Mailing address: Department of
Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319)
335-7780. Fax: (319) 335-9006. E-mail:
ldaniels{at}blue.weeg.uiowa.edu.
Present address: Department of Microbiology, University of
Illinois, Urbana, IL 61801.
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