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J Bacteriol, April 1998, p. 2237-2243, Vol. 180, No. 8
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The tfdK Gene Product Facilitates Uptake of 2,4-Dichlorophenoxyacetate by Ralstonia eutropha JMP134(pJP4)

Johan H. J. Leveau, Alexander J. B. Zehnder, and Jan Roelof van der Meer*

Swiss Federal Institute for Environmental Science and Technology (EAWAG) and Swiss Federal Institute for Technology (ETH), CH-8600 Dübendorf, Switzerland

Received 5 September 1997/Accepted 16 February 1998

Uptake of 2,4-dichlorophenoxyacetate (2,4-D) by Ralstonia eutropha JMP134(pJP4) was studied and shown to be an energy-dependent process. The uptake system was inducible with 2,4-D and followed saturation kinetics in a concentration range of up to 60 µM, implying the involvement of a protein in the transport process. We identified an open reading frame on plasmid pJP4, which was designated tfdK, whose translation product TfdK was highly hydrophobic and showed resemblance to transport proteins of the major facilitator superfamily. An interruption of the tfdK gene on plasmid pJP4 decimated 2,4-D uptake rates, which implies a role for TfdK in uptake. A tfdA mutant, which was blocked in the first step of 2,4-D metabolism, still took up 2,4-D. A mathematical model describing TfdK as an active transporter at low micromolar concentrations fitted the observed uptake data best.

* Corresponding author. Mailing address: Swiss Federal Institute for Environmental Science and Technology (EAWAG) and Swiss Federal Institute for Technology (ETH), Ueberlandstrasse 133, CH-8600 Dübendorf, Switzerland. Phone: 41-1-823-5438. Fax: 41-1-823-5547. E-mail: vdmeer{at}eawag.ch.




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