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J Bacteriol, April 1998, p. 2237-2243, Vol. 180, No. 8
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The tfdK Gene Product Facilitates Uptake of
2,4-Dichlorophenoxyacetate by Ralstonia eutropha
JMP134(pJP4)
Johan H. J.
Leveau,
Alexander J. B.
Zehnder, and
Jan Roelof
van der
Meer*
Swiss Federal Institute for Environmental
Science and Technology (EAWAG) and Swiss Federal Institute for
Technology (ETH), CH-8600 Dübendorf, Switzerland
Received 5 September 1997/Accepted 16 February 1998
Uptake of 2,4-dichlorophenoxyacetate (2,4-D) by Ralstonia
eutropha JMP134(pJP4) was studied and shown to be an
energy-dependent process. The uptake system was inducible with 2,4-D
and followed saturation kinetics in a concentration range
of up to 60 µM, implying the involvement of a protein in the
transport process. We identified an open reading frame on plasmid pJP4,
which was designated tfdK, whose translation product TfdK
was highly hydrophobic and showed resemblance to transport proteins of
the major facilitator superfamily. An interruption of the
tfdK gene on plasmid pJP4 decimated 2,4-D uptake rates,
which implies a role for TfdK in uptake. A tfdA mutant,
which was blocked in the first step of 2,4-D metabolism, still took up
2,4-D. A mathematical model describing TfdK as an active transporter at
low micromolar concentrations fitted the observed uptake data best.
*
Corresponding author. Mailing address: Swiss Federal
Institute for Environmental Science and Technology (EAWAG) and Swiss Federal Institute for Technology (ETH), Ueberlandstrasse 133, CH-8600
Dübendorf, Switzerland. Phone: 41-1-823-5438. Fax:
41-1-823-5547. E-mail: vdmeer{at}eawag.ch.
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