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J Bacteriol, April 1998, p. 2244-2247, Vol. 180, No. 8
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Erwinia amylovora Secretes DspE, a Pathogenicity Factor and Functional AvrE Homolog, through the Hrp (Type III Secretion) Pathway

Adam J. Bogdanove, David W. Bauer, and Steven V. Beer^*

Department of Plant Pathology, Cornell University, Ithaca, New York 14853

Received 20 October 1997/Accepted 11 February 1998

Erwinia amylovora was shown to secrete DspE, a pathogenicity factor of 198 kDa and a functional homolog of AvrE of Pseudomonas syringae pv. tomato. DspE was identified among the supernatant proteins isolated from cultures grown in an hrp gene-inducing minimal medium by immunodetection with a DspE-specific antiserum. Secretion required an intact Hrp pathway.

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^* Corresponding author. Mailing address: Department of Plant Pathology, Cornell University, 334 Plant Science Building, Ithaca, NY 14853-4203. Phone: (607) 255-7870. Fax: (607) 255-4471. E-mail: svb1{at}cornell.edu.

Present address: Department of Agronomy, Purdue University, West Lafayette, IN 47907-1150.

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