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J Bacteriol, April 1998, p. 2253-2256, Vol. 180, No. 8
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Physiological Regulation of the Derepressible Phosphate Transporter in Saccharomyces cerevisiae

Paula Martinez,1 Renata Zvyagilskaya,2 Peter Allard,3 and Bengt L. Persson1,4,*

A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, 117071, Moscow, Russia,2 and Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, University of Stockholm, S-106 91 Stockholm,1 Center for Structural Biochemistry, Novum, S-141 57 Huddinge,3 and Department of Engineering and Natural Sciences, University of Växjö, S-351 95 Växjö,4 Sweden

Received 23 October 1997/Accepted 10 February 1998

The extracellular phosphate concentration permissive for the expression of different amounts of the active high-affinity Pho84 phosphate transporter in the plasma membrane as well as the PHO84 messenger RNA levels in low-phosphate-grown Saccharomyces cerevisiae cells is very narrow and essential for a tight regulation of the transporter. The Pho84 transporter undergoes a rapid degradation once the supply of phosphate and/or carbon source is exhausted.


* Corresponding author. Mailing address: Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, University of Stockholm, S-106 91 Stockholm, Sweden. Phone: 46(8)162469. Fax: 46(8)153679. E-mail: Bengt_P{at}biokemi.su.se.




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