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J Bacteriol, May 1998, p. 2298-2305, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Nucleotide Sequence and Spatiotemporal Expression of the Vibrio cholerae vieSAB Genes during Infection

Sang Ho Lee,1 Michael J. Angelichio,1 John J. Mekalanos,2 and Andrew Camilli1,*

Department of Molecular Biology & Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111,1 and Department of Microbiology & Molecular Genetics, Harvard Medical School, Boston, Massachusetts 021152

Received 25 November 1997/Accepted 25 February 1998

The iviVII gene of Vibrio cholerae was previously identified by a screen for genes induced during intestinal infection. In the present study, nucleotide sequence analysis revealed that iviVII is a 1,659-bp open reading frame, herein designated vieB, that is predicted to be last in a tricistronic operon (vieSAB). The deduced amino acid sequence of VieS exhibited similarity to the sensor kinase component, and those of VieA and VieB were similar to the response regulator components, respectively, of the two-component signal transduction family. Analysis of transcriptional fusions to a site-specific DNA recombinase reporter, tnpR, revealed that vieS and vieA are transcribed during in vitro growth in a vieAB-independent and vieA-dependent manner, respectively. In contrast, transcription of vieB occurred exclusively during infection and was not dependent upon VieB. We conclude that the vieSAB genes are differentially regulated, at least during laboratory growth. Use of a V. cholerae strain harboring a vieB::tnpR transcriptional fusion allowed the kinetics and location of vieB expression within the intestine to be determined. We found that vieB transcription is induced shortly after infection of the proximal and mid-small intestine.

* Corresponding author. Mailing address: Department of Molecular Biology & Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6653. Fax: (617) 636-0337. E-mail: acamilli{at}opal.tufts.edu.

J Bacteriol, May 1998, p. 2298-2305, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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