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J Bacteriol, May 1998, p. 2298-2305, Vol. 180, No. 9
Department of Molecular Biology & Microbiology, Tufts University School of Medicine, Boston,
Massachusetts 02111,1 and
Department of
Microbiology & Molecular Genetics, Harvard Medical School, Boston,
Massachusetts 021152
Received 25 November 1997/Accepted 25 February 1998
The iviVII gene of Vibrio cholerae was
previously identified by a screen for genes induced during intestinal
infection. In the present study, nucleotide sequence analysis revealed
that iviVII is a 1,659-bp open reading frame, herein
designated vieB, that is predicted to be last in a
tricistronic operon (vieSAB). The deduced amino acid
sequence of VieS exhibited similarity to the sensor kinase component,
and those of VieA and VieB were similar to the response regulator
components, respectively, of the two-component signal transduction
family. Analysis of transcriptional fusions to a site-specific DNA
recombinase reporter, tnpR, revealed that vieS
and vieA are transcribed during in vitro growth in a
vieAB-independent and vieA-dependent manner,
respectively. In contrast, transcription of vieB occurred
exclusively during infection and was not dependent upon VieB. We
conclude that the vieSAB genes are differentially regulated, at least during laboratory growth. Use of a V. cholerae strain harboring a
vieB::tnpR transcriptional fusion
allowed the kinetics and location of vieB expression within
the intestine to be determined. We found that vieB
transcription is induced shortly after infection of the proximal and
mid-small intestine.
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Nucleotide Sequence and Spatiotemporal Expression
of the Vibrio cholerae vieSAB Genes during
Infection
*
Corresponding author. Mailing address: Department of
Molecular Biology & Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6653. Fax: (617)
636-0337. E-mail: acamilli{at}opal.tufts.edu.
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