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J Bacteriol, May 1998, p. 2350-2358, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Evolution of Mycoplasma capricolum subsp. capripneumoniae Strains, Based on Polymorphisms in the 16S rRNA Genes

Bertil Pettersson,1 Göran Bölske,2 François Thiaucourt,3 Mathias Uhlén,1 and Karl-Erik Johansson2,4,*

Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-100 44 Stockholm,1 Department of Bacteriology, National Veterinary Institute,2 and Department of Veterinary Microbiology, Swedish University of Agricultural Sciences,4 S-750 07 Uppsala, Sweden, and Département d'élevage et de médecine véterinaire, Centre de coopération internationale en recherche agronomique pour le dévelopment, F-34032 Montpellier, France3

Received 18 November 1997/Accepted 23 February 1998

Mycoplasma capricolum subsp. capripneumoniae belongs to the so-called Mycoplasma mycoides cluster and is the causal agent of contagious caprine pleuropneumonia (CCPP). All members of the M. mycoides cluster have two rRNA operons. The sequences of the 16S rRNA genes of both rRNA operons from 20 strains of M. capricolum subsp. capripneumoniae of different geographical origins in Africa and Asia were determined. Nucleotide differences which were present in only one of the two operons (polymorphisms) were detected in 24 positions. The polymorphisms were not randomly distributed in the 16S rRNA genes, and some of them were found in regions of low evolutionary variability. Interestingly, 11 polymorphisms were found in all the M. capricolum subsp. capripneumoniae strains, thus defining a putative ancestor. A sequence length difference between the 16S rRNA genes in a poly(A) region and 12 additional polymorphisms were found in only one or some of the strains. A phylogenetic tree was constructed by comparative analysis of the polymorphisms, and this tree revealed two distinct lines of descent. The nucleotide substitution rate of strains within line II was up to 50% higher than within line I. A tree was also constructed from individual operonal 16S rRNA sequences, and the sequences of the two operons were found to form two distinct clades. The topologies of both clades were strikingly similar, which supports the use of 16S rRNA sequence data from homologous operons for phylogenetic studies. The strain-specific polymorphism patterns of the 16S rRNA genes of M. capricolum subsp. capripneumoniae may be used as epidemiological markers for CCPP.

* Corresponding author. Mailing address: Department of Bacteriology, National Veterinary Institute, P.O. Box 7073, S-750 07 Uppsala, Sweden. Phone: 46 18 67 40 00. Fax: 46 18 30 91 62. E-mail: Kaggen{at}sva.se.

J Bacteriol, May 1998, p. 2350-2358, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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