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J Bacteriol, May 1998, p. 2359-2366, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mutational Analysis of the Chlamydia trachomatis rRNA P1 Promoter Defines Four Regions Important for Transcription In Vitro

Ming Tan,1,dagger Tamas Gaal,2 Richard L. Gourse,2 and Joanne N. Engel1,*

Department of Medicine, University of California, San Francisco, San Francisco, California 94143-0654,1 and Department of Bacteriology, University of Wisconsin---Madison, Madison, Wisconsin 537062

Received 29 January 1998/Accepted 6 March 1998

We have characterized the Chlamydia trachomatis ribosomal promoter, rRNA P1, by measuring the effect of substitutions and deletions on in vitro transcription with partially purified C. trachomatis RNA polymerase. Our analyses indicate that rRNA P1 contains potential -10 and -35 elements, analogous to Escherichia coli promoters recognized by E-sigma 70. We identified a novel AT-rich region immediately downstream of the -35 region. The effect of this region was specific for C. trachomatis RNA polymerase and strongly attenuated by single G or C substitutions. Upstream of the -35 region was an AT-rich sequence that enhanced transcription by C. trachomatis and E. coli RNA polymerases. We propose that this region functions as an UP element.

* Corresponding author. Mailing address: Box 0654, Division of Infectious Disease, Department of Medicine, University of California, San Francisco, San Francisco, CA 94143-0654. Phone: (415) 476-7355. Fax: (415) 476-9364. E-mail: joanne_engel{at}quickmail.ucsf.edu.

dagger Present address: Departments of Microbiology & Molecular Genetics and Medicine, University of California, Irvine, Irvine, CA 92697-4025.

J Bacteriol, May 1998, p. 2359-2366, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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