This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collier, L. S.
Right arrow Articles by Neidle, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collier, L. S.
Right arrow Articles by Neidle, E. L.

 Previous Article  |  Next Article 

J Bacteriol, May 1998, p. 2493-2501, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of Benzoate Degradation in Acinetobacter sp. Strain ADP1 by BenM, a LysR-Type Transcriptional Activator

Lauren S. Collier,1 George L. Gaines III,2 and Ellen L. Neidle1,*

Department of Microbiology, University of Georgia, Athens, Georgia 30602-2605,1 and Isogenetics, Inc., Chicago, Illinois 606122

Received 24 October 1997/Accepted 27 February 1998

In Acinetobacter sp. strain ADP1, benzoate degradation requires the ben genes for converting benzoate to catechol and the cat genes for degrading catechol. Here we describe a novel transcriptional activator, BenM, that regulates the chromosomal ben and cat genes. BenM is homologous to CatM, a LysR-type transcriptional activator of the cat genes. Unusual regulatory features of this system include the abilities of both BenM and CatM to recognize the same inducer, cis,cis-muconate, and to regulate some of the same genes, such as catA and catB. Unlike CatM, BenM responded to benzoate. Benzoate together with cis,cis-muconate increased the BenM-dependent expression of the benABCDE operon synergistically. CatM was not required for this synergism, nor did CatM regulate the expression of a chromosomal benA::lacZ transcriptional fusion. BenM-mediated regulation differs significantly from that of the TOL plasmid-encoded conversion of benzoate to catechol in pseudomonads. The benM gene is immediately upstream of, and divergently transcribed from, benA, and a possible DNA binding site for BenM was identified between the two coding regions. Two mutations in the predicted operator/promoter region rendered ben gene expression either constitutive or inducible by cis,cis-muconate but not benzoate. Mutants lacking BenM, CatM, or both of these regulators degraded aromatic compounds at different rates, and the levels of intermediary metabolites that accumulated depended on the genetic background. These studies indicated that BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. In a catM-disrupted strain, BenM was able to induce higher levels of catA expression than catB expression.

* Corresponding author. Mailing address: Department of Microbiology, University of Georgia, 527 Biological Sciences Building, Athens, GA 30602-2605. Phone: (706) 542-2852. Fax: (706) 542-2674. E-mail: eneidle{at}uga.cc.uga.edu.

J Bacteriol, May 1998, p. 2493-2501, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maddocks, S. E., Oyston, P. C. F. (2008). Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Microbiology 154: 3609-3623 [Abstract] [Full Text]  
  • Fischer, R., Bleichrodt, F. S., Gerischer, U. C. (2008). Aromatic degradative pathways in Acinetobacter baylyi underlie carbon catabolite repression. Microbiology 154: 3095-3103 [Abstract] [Full Text]  
  • Putrins, M., Tover, A., Tegova, R., Saks, U., Kivisaar, M. (2007). Study of factors which negatively affect expression of the phenol degradation operon pheBA in Pseudomonas putida. Microbiology 153: 1860-1871 [Abstract] [Full Text]  
  • Hare, J. M., Perkins, S. N., Gregg-Jolly, L. A. (2006). A Constitutively Expressed, Truncated umuDC Operon Regulates the recA-Dependent DNA Damage Induction of a Gene in Acinetobacter baylyi Strain ADP1.. Appl. Environ. Microbiol. 72: 4036-4043 [Abstract] [Full Text]  
  • Ezezika, O. C., Collier-Hyams, L. S., Dale, H. A., Burk, A. C., Neidle, E. L. (2006). CatM Regulation of the benABCDE Operon: Functional Divergence of Two LysR-Type Paralogs in Acinetobacter baylyi ADP1.. Appl. Environ. Microbiol. 72: 1749-1758 [Abstract] [Full Text]  
  • Palacios, S., Escalante-Semerena, J. C. (2004). 2-Methylcitrate-dependent activation of the propionate catabolic operon (prpBCDE) of Salmonella enterica by the PrpR protein. Microbiology 150: 3877-3887 [Abstract] [Full Text]  
  • Tropel, D., van der Meer, J. R. (2004). Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. Microbiol. Mol. Biol. Rev. 68: 474-500 [Abstract] [Full Text]  
  • Buchan, A., Neidle, E. L., Moran, M. A. (2004). Diverse Organization of Genes of the {beta}-Ketoadipate Pathway in Members of the Marine Roseobacter Lineage. Appl. Environ. Microbiol. 70: 1658-1668 [Abstract] [Full Text]  
  • Clark, T. J., Phillips, R. S., Bundy, B. M., Momany, C., Neidle, E. L. (2004). Benzoate Decreases the Binding of cis,cis-Muconate to the BenM Regulator despite the Synergistic Effect of Both Compounds on Transcriptional Activation. J. Bacteriol. 186: 1200-1204 [Abstract] [Full Text]  
  • Chang, H.-K., Mohseni, P., Zylstra, G. J. (2003). Characterization and Regulation of the Genes for a Novel Anthranilate 1,2-Dioxygenase from Burkholderia cepacia DBO1. J. Bacteriol. 185: 5871-5881 [Abstract] [Full Text]  
  • Tralau, T., Mampel, J., Cook, A. M., Ruff, J. (2003). Characterization of TsaR, an Oxygen-Sensitive LysR-Type Regulator for the Degradation of p-Toluenesulfonate in Comamonas testosteroni T-2. Appl. Environ. Microbiol. 69: 2298-2305 [Abstract] [Full Text]  
  • Brzostowicz, P. C., Reams, A. B., Clark, T. J., Neidle, E. L. (2003). Transcriptional Cross-Regulation of the Catechol and Protocatechuate Branches of the {beta}-Ketoadipate Pathway Contributes to Carbon Source-Dependent Expression of the Acinetobacter sp. Strain ADP1 pobA Gene. Appl. Environ. Microbiol. 69: 1598-1606 [Abstract] [Full Text]  
  • Cai, M., Xun, L. (2002). Organization and Regulation of Pentachlorophenol-Degrading Genes in Sphingobium chlorophenolicum ATCC 39723. J. Bacteriol. 184: 4672-4680 [Abstract] [Full Text]  
  • Bundy, B. M., Collier, L. S., Hoover, T. R., Neidle, E. L. (2002). Synergistic transcriptional activation by one regulatory protein in response to two metabolites. Proc. Natl. Acad. Sci. USA 99: 7693-7698 [Abstract] [Full Text]  
  • Miyauchi, K., Lee, H.-S., Fukuda, M., Takagi, M., Nagata, Y. (2002). Cloning and Characterization of linR, Involved in Regulation of the Downstream Pathway for {gamma}-Hexachlorocyclohexane Degradation in Sphingomonas paucimobilis UT26. Appl. Environ. Microbiol. 68: 1803-1807 [Abstract] [Full Text]  
  • Tichi, M. A., Tabita, F. R. (2002). Metabolic Signals That Lead to Control of CBB Gene Expression in Rhodobacter capsulatus. J. Bacteriol. 184: 1905-1915 [Abstract] [Full Text]  
  • Popp, R., Kohl, T., Patz, P., Trautwein, G., Gerischer, U. (2002). Differential DNA Binding of Transcriptional Regulator PcaU from Acinetobacter sp. Strain ADP1. J. Bacteriol. 184: 1988-1997 [Abstract] [Full Text]  
  • Clark, T. J., Momany, C., Neidle, E. L. (2002). The benPK operon, proposed to play a role in transport, is part of a regulon for benzoate catabolism in Acinetobacter sp. strain ADP1. Microbiology 148: 1213-1223 [Abstract] [Full Text]  
  • Gobel, M., Kassel-Cati, K., Schmidt, E., Reineke, W. (2002). Degradation of Aromatics and Chloroaromatics by Pseudomonas sp. Strain B13: Cloning, Characterization, and Analysis of Sequences Encoding 3-Oxoadipate:Succinyl-Coenzyme A (CoA) Transferase and 3-Oxoadipyl-CoA Thiolase. J. Bacteriol. 184: 216-223 [Abstract] [Full Text]  
  • Kitagawa, W., Miyauchi, K., Masai, E., Fukuda, M. (2001). Cloning and Characterization of Benzoate Catabolic Genes in the Gram-Positive Polychlorinated Biphenyl Degrader Rhodococcus sp. Strain RHA1. J. Bacteriol. 183: 6598-6606 [Abstract] [Full Text]  
  • Jones, R. M., Williams, P. A. (2001). areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a {sigma}54-Dependent Regulator. J. Bacteriol. 183: 405-409 [Abstract] [Full Text]  
  • Francisco, P. B. Jr, Ogawa, N., Suzuki, K., Miyashita, K. (2001). The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates. Microbiology 147: 121-133 [Abstract] [Full Text]  
  • Cosper, N. J., Collier, L. S., Clark, T. J., Scott, R. A., Neidle, E. L. (2000). Mutations in catB, the Gene Encoding Muconate Cycloisomerase, Activate Transcription of the Distal ben Genes and Contribute to a Complex Regulatory Circuit in Acinetobacter sp. Strain ADP1. J. Bacteriol. 182: 7044-7052 [Abstract] [Full Text]  
  • Cowles, C. E., Nichols, N. N., Harwood, C. S. (2000). BenR, a XylS Homologue, Regulates Three Different Pathways of Aromatic Acid Degradation in Pseudomonas putida. J. Bacteriol. 182: 6339-6346 [Abstract] [Full Text]  
  • Jones, R. M., Pagmantidis, V., Williams, P. A. (2000). sal Genes Determining the Catabolism of Salicylate Esters Are Part of a Supraoperonic Cluster of Catabolic Genes in Acinetobacter sp. Strain ADP1. J. Bacteriol. 182: 2018-2025 [Abstract] [Full Text]  
  • Park, H.-S., Kim, H.-S. (2000). Identification and Characterization of the Nitrobenzene Catabolic Plasmids pNB1 and pNB2 in Pseudomonas putida HS12. J. Bacteriol. 182: 573-580 [Abstract] [Full Text]  
  • D'Argenio, D. A., Vetting, M. W., Ohlendorf, D. H., Ornston, L. N. (1999). Substitution, Insertion, Deletion, Suppression, and Altered Substrate Specificity in Functional Protocatechuate 3,4-Dioxygenases. J. Bacteriol. 181: 6478-6487 [Abstract] [Full Text]  
  • Jones, R. M., Collier, L. S., Neidle, E. L., Williams, P. A. (1999). areABC Genes Determine the Catabolism of Aryl Esters in Acinetobacter sp. Strain ADP1. J. Bacteriol. 181: 4568-4575 [Abstract] [Full Text]  
  • Bundy, B. M., Campbell, A. L., Neidle, E. L. (1998). Similarities between the antABC-Encoded Anthranilate Dioxygenase and the benABC-Encoded Benzoate Dioxygenase of Acinetobacter sp. Strain ADP1. J. Bacteriol. 180: 4466-4474 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»