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J Bacteriol, May 1998, p. 2556-2559, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Saccharomyces cerevisiae YCC5 (YCL025c) Gene Encodes an Amino Acid Permease, Agp1, Which Transports Asparagine and Glutamine

James L. Schreve, Jennifer K. Sin, and Jinnie M. Garrett*

Department of Biology, Hamilton College, Clinton, New York 13323

Received 20 June 1997/Accepted 18 February 1998

The yeast YCC5 gene encodes a putative amino acid permease and is homologous to GNP1 (encoding a high-affinity glutamine permease). Using strains with disruptions in the genes for multiple permeases, we demonstrated that Ycc5 (which we have renamed Agp1) is involved in the transport of asparagine and glutamine, performed a kinetic analysis of this activity, and showed that AGP1 expression is subject to nitrogen repression.

* Corresponding author. Mailing address: Department of Biology, Hamilton College, 198 College Hill Rd., Clinton, NY 13323. Phone: (315) 859-4716. Fax: (315) 859-4807. E-mail: jgarrett{at}hamilton.edu.

J Bacteriol, May 1998, p. 2556-2559, Vol. 180, No. 9
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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