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Journal of Bacteriology, January 1999, p. 117-125, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Chromosome Map of Xanthomonas campestris pv. campestris 17 with Locations of Genes Involved in Xanthan Gum Synthesis and Yellow Pigmentation

Yi-Hsiung Tseng,* Ka-Tim Choy, Chih-Hsin Hung, Nien-Tsung Lin,dagger Jane-Yu Liu, Chih-Hong Lou, Bih-Ying Yang,Dagger Fu-Shyan Wen, Shu-Fen Weng, and Jung-Rung Wu§

Institute of Molecular Biology and Department of Botany, National Chung Hsing University, Taichung 402, Taiwan

Received 30 July 1998/Accepted 20 October 1998

No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths in these digests. Based on the data obtained from partial digestion and Southern hybridization using probes common to pairs of the overlapping fragments or prepared from linking fragments, a circular physical map bearing the PacI, PmeI, and SwaI sites was constructed for the X. campestris pv. campestris 17 chromosome. Locations of eight eps loci involved in exopolysaccharide (xanthan gum) synthesis, two rrn operons each possessing an unique I-CeuI site, one pig cluster required for yellow pigmentation, and nine auxotrophic markers were determined, using mutants isolated by mutagenesis with Tn5(pfm)CmKm. This transposon contains a polylinker with sites for several rare-cutting restriction endonucleases located between the chloramphenicol resistance and kanamycin resistance (Kmr) genes, which upon insertion introduced additional sites into the chromosome. The recA and tdh genes, with known sequences, were mapped by tagging with the polylinker-Kmr segment from Tn5(pfm)CmKm. This is the first map for X. campestris and would be useful for genetic studies of this and related Xanthomonas species.

* Corresponding author. Mailing address: Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan. Phone: 886-4-285-1885. Fax: 886-4-287-4879. E-mail: yhtseng{at}dragon.nchu.edu.tw.

dagger Present address: Department of Microbiology, Tzu-Chi College of Medicine and Humanities, Hualien 970, Taiwan.

Dagger Present address: Biotechnology Center, University of Connecticut, Storrs, CT 06269-3149.

§ Present address: Center for Human Genome Research, Los Alamos National Laboratory, Los Alamos, NM 87544.

Journal of Bacteriology, January 1999, p. 117-125, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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