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Journal of Bacteriology, January 1999, p. 149-152, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

3-Nitroadipate, a Metabolic Intermediate for Mineralization of 2,4-Dinitrophenol by a New Strain of a Rhodococcus Species

Rafael Blasco,1,* Edward Moore,2 Victor Wray,2 Dietmar Pieper,2 Kenneth Timmis,2 and Francisco Castillo1

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, 14071 Córdoba, Spain,1 and GBF (National Research Center for Biotechnology), D-38124 Braunschweig, Germany2

Received 1 June 1998/Accepted 21 October 1998

The bacterial strain RB1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16S rRNA gene sequence comparison, as a Rhodococcus species closely related to Rhodococcus opacus. Rhodococcus sp. strain RB1 degrades 2,4-dinitrophenol, releasing the two nitro groups from the compound as nitrite. The release of nitro groups from 2,4-dinitrophenol occurs in two steps. First, the 2-nitro group is removed as nitrite, with the production of an aliphatic nitro compound identified by 1H nuclear magnetic resonance and mass spectrometry as 3-nitroadipate. Then, this metabolic derivative is further metabolized, releasing its nitro group as nitrite. Full nitrite assimilation upon reduction to ammonia requires that an additional carbon source be supplied to the medium.

* Corresponding author. Mailing address: Departamento de Bioquímica, Facultad de Ciencias, Avenida San Alberto Magno s/n, 14071 Cordoba, Spain. Phone: 34 57218592. Fax: 34 57218606. E-mail: bb1blplr{at}uco.es.

Journal of Bacteriology, January 1999, p. 149-152, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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