Recruitment of ZipA to the Septal Ring of Escherichia coli Is Dependent on FtsZ and Independent of FtsA

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Journal of Bacteriology, January 1999, p. 167-176, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Recruitment of ZipA to the Septal Ring of Escherichia coli Is Dependent on FtsZ and Independent of FtsA

Cynthia A. Hale and Piet A. J. de Boer^*

Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960

Received 13 July 1998/Accepted 26 October 1998

Cell division in prokaryotes is mediated by the septal ring. In Escherichia coli, this organelle consists of several essentialdivision proteins, including FtsZ, FtsA, and ZipA. To gain moreinsight into how the structure is assembled, we studied the interdependenceof FtsZ, FtsA, and ZipA localization using both immunofluorescenceand Gfp tagging techniques. To this end, we constructed a setof strains allowing us to determine the cellular location of eachof these three proteins in cells from which one of the other twohad been specifically depleted. Our results show that ZipA failsto accumulate in a ring shape in the absence of FtsZ. Conversely,depletion of ZipA does not abolish formation of FtsZ rings butleads to a significant reduction in the number of rings per unitof cell mass. In addition, ZipA does not appear to require FtsAfor assembly into the septal ring and vice versa. It is suggestedthat septal ring formation starts by assembly of the FtsZ ring,after which ZipA and FtsA join this structure in a mutually independentfashion through direct interactions with the FtsZprotein.

^* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, School of Medicine, Case WesternReserve University, 10900 Euclid Ave., Cleveland, OH 44106-4960.Phone: (216) 368-1697. Fax: (216) 368-3055. E-mail: pad5{at}po.cwru.edu.

Journal of Bacteriology, January 1999, p. 167-176, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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