This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Howell, D. M.
Right arrow Articles by White, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Howell, D. M.
Right arrow Articles by White, R. H.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 1999, p. 331-333, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

(R)-Citramalate Synthase in Methanogenic Archaea

David M. Howell, Huimin Xu, and Robert H. White*

Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308

Received 18 June 1998/Accepted 19 October 1998

The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.

* Corresponding author. Mailing address: Department of Biochemistry, College of Agriculture and Life Science, Virginia Polytechnic Institute and State University, 111 Engel Hall, Blacksburg, VA 24061-0308. Phone: (540) 231-6605. Fax: (540) 231-9070. E-mail: rhwhite{at}vt.edu.

Journal of Bacteriology, January 1999, p. 331-333, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Atsumi, S., Liao, J. C. (2008). Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli. Appl. Environ. Microbiol. 74: 7802-7808 [Abstract] [Full Text]  
  • Risso, C., Van Dien, S. J., Orloff, A., Lovley, D. R., Coppi, M. V. (2008). Elucidation of an Alternate Isoleucine Biosynthesis Pathway in Geobacter sulfurreducens. J. Bacteriol. 190: 2266-2274 [Abstract] [Full Text]  
  • Wu, J., Tao, Y., Zhang, M., Howard, M. H., Gutteridge, S., Ding, J. (2007). Crystal Structures of Saccharomyces cerevisiae N-Myristoyltransferase with Bound Myristoyl-CoA and Inhibitors Reveal the Functional Roles of the N-terminal Region. J. Biol. Chem. 282: 22185-22194 [Abstract] [Full Text]  
  • Drevland, R. M., Waheed, A., Graham, D. E. (2007). Enzymology and Evolution of the Pyruvate Pathway to 2-Oxobutyrate in Methanocaldococcus jannaschii. J. Bacteriol. 189: 4391-4400 [Abstract] [Full Text]  
  • Jahn, U., Huber, H., Eisenreich, W., Hugler, M., Fuchs, G. (2007). Insights into the Autotrophic CO2 Fixation Pathway of the Archaeon Ignicoccus hospitalis: Comprehensive Analysis of the Central Carbon Metabolism. J. Bacteriol. 189: 4108-4119 [Abstract] [Full Text]  
  • Li, F., Hagemeier, C. H., Seedorf, H., Gottschalk, G., Thauer, R. K. (2007). Re-Citrate Synthase from Clostridium kluyveri Is Phylogenetically Related to Homocitrate Synthase and Isopropylmalate Synthase Rather Than to Si-Citrate Synthase. J. Bacteriol. 189: 4299-4304 [Abstract] [Full Text]  
  • Friedmann, S., Alber, B. E., Fuchs, G. (2007). Properties of R-Citramalyl-Coenzyme A Lyase and Its Role in the Autotrophic 3-Hydroxypropionate Cycle of Chloroflexus aurantiacus. J. Bacteriol. 189: 2906-2914 [Abstract] [Full Text]  
  • Hendrickson, E. L., Kaul, R., Zhou, Y., Bovee, D., Chapman, P., Chung, J., Conway de Macario, E., Dodsworth, J. A., Gillett, W., Graham, D. E., Hackett, M., Haydock, A. K., Kang, A., Land, M. L., Levy, R., Lie, T. J., Major, T. A., Moore, B. C., Porat, I., Palmeiri, A., Rouse, G., Saenphimmachak, C., Soll, D., Van Dien, S., Wang, T., Whitman, W. B., Xia, Q., Zhang, Y., Larimer, F. W., Olson, M. V., Leigh, J. A. (2004). Complete Genome Sequence of the Genetically Tractable Hydrogenotrophic Methanogen Methanococcus maripaludis. J. Bacteriol. 186: 6956-6969 [Abstract] [Full Text]  
  • Xu, H., Zhang, Y., Guo, X., Ren, S., Staempfli, A. A., Chiao, J., Jiang, W., Zhao, G. (2004). Isoleucine Biosynthesis in Leptospira interrogans Serotype lai Strain 56601 Proceeds via a Threonine-Independent Pathway. J. Bacteriol. 186: 5400-5409 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»