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Journal of Bacteriology, January 1999, p. 347-352, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

APT1, but Not APT2, Codes for a Functional Adenine Phosphoribosyltransferase in Saccharomyces cerevisiae

Juan D. Alfonzo,1,dagger Timothy R. Crother,1 Maria L. Guetsova,2 Bertrand Daignan-Fornier,2 and Milton W. Taylor1,*

Department of Biology, Indiana University, Bloomington, Indiana 47405,1 and Institut de Biochimie et Genetique Cellulaires, Bordeaux, France2

Received 18 August 1998/Accepted 21 October 1998

The yeast Saccharomyces cerevisiae has two separate genes (APT1 and APT2) that encode two potentially different forms of adenine phosphoribosyltransferase (APRT). However, genetic analysis indicated that only APT1 could code for a complementing activity. Cloning and expression of both the APT1 and APT2 genes in Escherichia coli showed that although discrete proteins (APRT1 and APRT2) were made by these genes, only APRT1 had detectable APRT activity. Northern and Western blot analyses demonstrated that only APT1 was transcribed and translated under normal physiological conditions in yeast. Phylogenetic analysis revealed that APRT1 and APRT2 are evolutionary closely related and that they arise from a gene duplication event. We conclude that APT1 is the functional gene in S. cerevisiae and that APT2 is a pseudogene.

* Corresponding author. Mailing address: Department of Biology, Indiana University, Bloomington, IN 47405. Phone: (812) 855-3340. Fax: (812) 855-6705. E-mail: taylor{at}indiana.edu.

dagger Present address: Howard Hughes Medical Institute, MacDonald Research Laboratories, University of California at Los Angeles, Los Angeles, CA 90024.

Journal of Bacteriology, January 1999, p. 347-352, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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