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Journal of Bacteriology, January 1999, p. 78-82, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Two Distinct Mechanisms Cause Heterogeneity of 16S rRNA

Kumiko Ueda,1,dagger Tatsuji Seki,1 Takuji Kudo,2 Toshiomi Yoshida,1 and Masakazu Kataoka1,3,*

International Center for Biotechnology, Osaka University, Yamada-oka, Suita, Osaka 565,1 Japan Collection of Microorganisms, Institute of Physical and Chemical Research, Hirosawa, Wako, Saitama 351-01,2 and Mitsubishi-kasei Institute of Life Sciences, Minami-ooya, Machida, Tokyo 194-8511,3 Japan

Received 10 April 1998/Accepted 21 October 1998

To investigate the frequency of heterogeneity among the multiple 16S rRNA genes within a single microorganism, we determined directly the 120-bp nucleotide sequences containing the hypervariable alpha  region of the 16S rRNA gene from 475 Streptomyces strains. Display of the direct sequencing patterns revealed the existence of 136 heterogeneous loci among a total of 33 strains. The heterogeneous loci were detected only in the stem region designated helix 10. All of the substitutions conserved the relevant secondary structure. The 33 strains were divided into two groups: one group, including 22 strains, had less than two heterogeneous bases; the other group, including 11 strains, had five or more heterogeneous bases. The two groups were different in their combinations of heterogeneous bases. The former mainly contained transitional substitutions, and the latter was mainly composed of transversional substitutions, suggesting that at least two mechanisms, possibly misincorporation during DNA replication and horizontal gene transfer, cause rRNA heterogeneity.

* Corresponding author. Mailing address: Mitsubishi-kasei Institute of Life Sciences, Minami-ooya 11, Machida, Tokyo 194, Japan. Phone: 88-427-24-6283. Fax: 88-427-24-6314. E-mail: mars{at}libra.ls.m-kagaku.co.jp.

dagger Present address: Institute for Fermentation, Osaka, Juso-honmachi, Yodogawa-ku, Osaka 532, Japan.

Journal of Bacteriology, January 1999, p. 78-82, Vol. 181, No. 1
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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