This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alekshun, M. N.
Right arrow Articles by Levy, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alekshun, M. N.
Right arrow Articles by Levy, S. B.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 1999, p. 3303-3306, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of MarR Superrepressor Mutants

Michael N. Alekshun1,2 and Stuart B. Levy1,2,3,*

Center for Adaptation Genetics and Drug Resistance,1 and Departments of Molecular Biology and Microbiology2 and of Medicine,3 Tufts University School of Medicine, Boston, Massachusetts 02111

Received 14 December 1998/Accepted 21 March 1999

MarR negatively regulates expression of the multiple antibiotic resistance (mar) locus in Escherichia coli. Superrepressor mutants, generated in order to study regions of MarR required for function, exhibited altered inducer recognition properties in whole cells and increased DNA binding to marO in vitro. Mutations occurred in three areas of the relatively small MarR protein (144 amino acids). It is surmised that superrepression results from increased DNA binding activities of these mutant proteins.

* Corresponding author. Mailing address: Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6764. Fax: (617) 636-0458. E-mail: slevy{at}opal.tufts.edu.

Journal of Bacteriology, May 1999, p. 3303-3306, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kaczmarek, F. M., Dib-Hajj, F., Shang, W., Gootz, T. D. (2006). High-Level Carbapenem Resistance in a Klebsiella pneumoniae Clinical Isolate Is Due to the Combination of blaACT-1 {beta}-Lactamase Production, Porin OmpK35/36 Insertional Inactivation, and Down-Regulation of the Phosphate Transport Porin PhoE.. Antimicrob. Agents Chemother. 50: 3396-3406 [Abstract] [Full Text]  
  • Bina, X., Perreten, V., Levy, S. B. (2003). The Periplasmic Protein MppA Requires an Additional Mutated Locus To Repress marA Expression in Escherichia coli. J. Bacteriol. 185: 1465-1469 [Abstract] [Full Text]  
  • Providenti, M. A., Wyndham, R. C. (2001). Identification and Functional Characterization of CbaR, a MarR-Like Modulator of the cbaABC-Encoded Chlorobenzoate Catabolism Pathway. Appl. Environ. Microbiol. 67: 3530-3541 [Abstract] [Full Text]  
  • Balagué, C., Véscovi, E. G. (2001). Activation of Multiple Antibiotic Resistance in Uropathogenic Escherichia coli Strains by Aryloxoalcanoic Acid Compounds. Antimicrob. Agents Chemother. 45: 1815-1822 [Abstract] [Full Text]  
  • Alekshun, M. N., Levy, S. B. (1999). Alteration of the Repressor Activity of MarR, the Negative Regulator of the Escherichia coli marRAB Locus, by Multiple Chemicals In Vitro. J. Bacteriol. 181: 4669-4672 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»