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Journal of Bacteriology, May 1999, p. 3310-3316, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
NahY, a Catabolic Plasmid-Encoded Receptor Required
for Chemotaxis of Pseudomonas putida to the Aromatic
Hydrocarbon Naphthalene
Ann C.
Grimm
and
Caroline S.
Harwood*
Department of Microbiology, The University of
Iowa, Iowa City, Iowa 52242
Received 13 January 1999/Accepted 8 March 1999
Pseudomonas putida G7 exhibits chemotaxis to
naphthalene, but the molecular basis for this was not known. A new
gene, nahY, was found to be cotranscribed with
meta cleavage pathway genes on the NAH7 catabolic plasmid
for naphthalene degradation. The nahY gene encodes a
538-amino-acid protein with a membrane topology and a C-terminal region
that resemble those of chemotaxis transducer proteins. A P. putida G7 nahY mutant grew on naphthalene but was not
chemotactic to this aromatic hydrocarbon. The protein NahY thus appears
to function as a chemoreceptor for naphthalene or a related compound.
The presence of nahY on a catabolic plasmid implies that
chemotaxis may facilitate biodegradation.
*
Corresponding author. Mailing address: Department of
Microbiology, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7783. Fax: (319) 335-7679. E-mail:
caroline-harwood{at}uiowa.edu.

Present address: National Exposure Research Laboratory, U.S.
Environmental Protection Agency, Cincinnati, OH
45268.
Journal of Bacteriology, May 1999, p. 3310-3316, Vol. 181, No. 10
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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