This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dussurget, O.
Right arrow Articles by Smith, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dussurget, O.
Right arrow Articles by Smith, I.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 1999, p. 3402-3408, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transcriptional Control of the Iron-Responsive fxbA Gene by the Mycobacterial Regulator IdeRdagger

Olivier Dussurget,1,2 Juliano Timm,1 Manuel Gomez,1 Benjamin Gold,1,3 Shengwei Yu,4 Sue Z. Sabol,5 Randall K. Holmes,6 William R. Jacobs Jr.,4 and Issar Smith1,*

TB Center, Public Health Research Institute,1 and Department of Microbiology, New York University Medical Center,3 New York, New York 10016; UFR de Biochimie, Université Paris 7, 75251 Paris Cedex 05, France2; Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York 104614; Section of Gene Structure and Regulation, Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 208925; and Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 802626

Received 12 March 1998/Accepted 30 March 1999

Exochelin is the primary extracellular siderophore of Mycobacterium smegmatis, and the iron-regulated fxbA gene encodes a putative formyltransferase, an essential enzyme in the exochelin biosynthetic pathway (E. H. Fiss, Y. Yu, and W. R. Jacobs, Jr., Mol. Microbiol. 14:557-569, 1994). We investigated the regulation of fxbA by the mycobacterial IdeR, a homolog of the Corynebacterium diphtheriae iron regulator DtxR (M. P. Schmitt, M. Predich, L. Doukhan, I. Smith, and R. K. Holmes, Infect. Immun. 63:4284-4289, 1995). Gel mobility shift experiments showed that IdeR binds to the fxbA regulatory region in the presence of divalent metals. DNase I footprinting assays indicated that IdeR binding protects a 28-bp region containing a palindromic sequence of the fxbA promoter that was identified in primer extension assays. fxbA regulation was measured in M. smegmatis wild-type and ideR mutant strains containing fxbA promoter-lacZ fusions. These experiments confirmed that fxbA expression is negatively regulated by iron and showed that inactivation of ideR results in iron-independent expression of fxbA. However, the levels of its expression in the ideR mutant were approximately 50% lower than those in the wild-type strain under iron limitation, indicating an undefined positive role of IdeR in the regulation of fxbA.

* Corresponding author. Mailing address: TB Center, Public Health Research Institute, 455 First Ave., New York, NY 10016. Phone: (212) 578-0867. Fax: (212) 578-0804. E-mail: smitty{at}phri.nyu.edu.

dagger Publication no. 64 from the TB Center, Public Health Research Institute.

Journal of Bacteriology, June 1999, p. 3402-3408, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Goude, R., Amin, A. G., Chatterjee, D., Parish, T. (2008). The Critical Role of embC in Mycobacterium tuberculosis. J. Bacteriol. 190: 4335-4341 [Abstract] [Full Text]  
  • Liu, C., Mao, K., Zhang, M., Sun, Z., Hong, W., Li, C., Peng, B., Chang, Z. (2008). The SH3-like Domain Switches Its Interaction Partners to Modulate the Repression Activity of Mycobacterial Iron-dependent Transcription Regulator in Response to Metal Ion Fluctuations. J. Biol. Chem. 283: 2439-2453 [Abstract] [Full Text]  
  • Amin, A. G., Goude, R., Shi, L., Zhang, J., Chatterjee, D., Parish, T. (2008). EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology 154: 240-248 [Abstract] [Full Text]  
  • Schneider, C. Z., Parish, T., Basso, L. A., Santos, D. S. (2008). The Two Chorismate Mutases from both Mycobacterium tuberculosis and Mycobacterium smegmatis: Biochemical Analysis and Limited Regulation of Promoter Activity by Aromatic Amino Acids. J. Bacteriol. 190: 122-134 [Abstract] [Full Text]  
  • Pang, X., Vu, P., Byrd, T. F., Ghanny, S., Soteropoulos, P., Mukamolova, G. V., Wu, S., Samten, B., Howard, S. T. (2007). Evidence for complex interactions of stress-associated regulons in an mprAB deletion mutant of Mycobacterium tuberculosis. Microbiology 153: 1229-1242 [Abstract] [Full Text]  
  • Maciag, A., Dainese, E., Rodriguez, G. M., Milano, A., Provvedi, R., Pasca, M. R., Smith, I., Palu, G., Riccardi, G., Manganelli, R. (2007). Global Analysis of the Mycobacterium tuberculosis Zur (FurB) Regulon. J. Bacteriol. 189: 730-740 [Abstract] [Full Text]  
  • Pashley, C. A., Brown, A. C., Robertson, D., Parish, T. (2006). Identification of the Mycobacterium tuberculosis GlnE promoter and its response to nitrogen availability.. Microbiology 152: 2727-2734 [Abstract] [Full Text]  
  • Oram, D. M., Jacobson, A. D., Holmes, R. K. (2006). Transcription of the Contiguous sigB, dtxR, and galE Genes in Corynebacterium diphtheriae: Evidence for Multiple Transcripts and Regulation by Environmental Factors.. J. Bacteriol. 188: 2959-2973 [Abstract] [Full Text]  
  • Manabe, Y. C., Hatem, C. L., Kesavan, A. K., Durack, J., Murphy, J. R. (2005). Both Corynebacterium diphtheriae DtxR(E175K) and Mycobacterium tuberculosis IdeR(D177K) Are Dominant Positive Repressors of IdeR-Regulated Genes in M. tuberculosis. Infect. Immun. 73: 5988-5994 [Abstract] [Full Text]  
  • Miranda-CasoLuengo, R., Duffy, P. S., O'Connell, E. P., Graham, B. J., Mangan, M. W., Prescott, J. F., Meijer, W. G. (2005). The Iron-Regulated iupABC Operon Is Required for Saprophytic Growth of the Intracellular Pathogen Rhodococcus equi at Low Iron Concentrations. J. Bacteriol. 187: 3438-3444 [Abstract] [Full Text]  
  • Prakash, P., Yellaboina, S., Ranjan, A., Hasnain, S. E. (2005). Computational prediction and experimental verification of novel IdeR binding sites in the upstream sequences of Mycobacterium tuberculosis open reading frames. Bioinformatics 21: 2161-2166 [Abstract] [Full Text]  
  • Chou, C. J., Wisedchaisri, G., Monfeli, R. R., Oram, D. M., Holmes, R. K., Hol, W. G. J., Beeson, C. (2004). Functional Studies of the Mycobacterium tuberculosis Iron-dependent Regulator. J. Biol. Chem. 279: 53554-53561 [Abstract] [Full Text]  
  • Oram, D. M., Avdalovic, A., Holmes, R. K. (2004). Analysis of Genes That Encode DtxR-Like Transcriptional Regulators in Pathogenic and Saprophytic Corynebacterial Species. Infect. Immun. 72: 1885-1895 [Abstract] [Full Text]  
  • Moody, D. B., Young, D. C., Cheng, T.-Y., Rosat, J.-P., Roura-mir, C., O'Connor, P. B., Zajonc, D. M., Walz, A., Miller, M. J., Levery, S. B., Wilson, I. A., Costello, C. E., Brenner, M. B. (2004). T Cell Activation by Lipopeptide Antigens. Science 303: 527-531 [Abstract] [Full Text]  
  • Parish, T. (2003). Starvation Survival Response of Mycobacterium tuberculosis. J. Bacteriol. 185: 6702-6706 [Abstract] [Full Text]  
  • Recchi, C., Sclavi, B., Rauzier, J., Gicquel, B., Reyrat, J.-M. (2003). Mycobacterium tuberculosis Rv1395 Is a Class III Transcriptional Regulator of the AraC Family Involved in Cytochrome P450 Regulation. J. Biol. Chem. 278: 33763-33773 [Abstract] [Full Text]  
  • Parish, T., Smith, D. A., Roberts, G., Betts, J., Stoker, N. G. (2003). The senX3-regX3 two-component regulatory system of Mycobacterium tuberculosis is required for virulence. Microbiology 149: 1423-1435 [Abstract] [Full Text]  
  • Collins, D. M., De Zoete, M., Cavaignac, S. M. (2002). Mycobacterium avium subsp. paratuberculosis Strains from Cattle and Sheep Can Be Distinguished by a PCR Test Based on a Novel DNA Sequence Difference. J. Clin. Microbiol. 40: 4760-4762 [Abstract] [Full Text]  
  • Oetjen, J., Fives-Taylor, P., Froeliger, E. H. (2002). The Divergently Transcribed Streptococcus parasanguis Virulence-Associated fimA Operon Encoding an Mn2+-Responsive Metal Transporter and pepO Encoding a Zinc Metallopeptidase Are Not Coordinately Regulated. Infect. Immun. 70: 5706-5714 [Abstract] [Full Text]  
  • Howard, S. T., Byrd, T. F., Lyons, C. R. (2002). A polymorphic region in Mycobacterium abscessus contains a novel insertion sequence element. Microbiology 148: 2987-2996 [Abstract] [Full Text]  
  • Rodriguez, G. M., Voskuil, M. I., Gold, B., Schoolnik, G. K., Smith, I. (2002). ideR, an Essential Gene in Mycobacterium tuberculosis: Role of IdeR in Iron-Dependent Gene Expression, Iron Metabolism, and Oxidative Stress Response. Infect. Immun. 70: 3371-3381 [Abstract] [Full Text]  
  • Benoit, S., Benachour, A., Taouji, S., Auffray, Y., Hartke, A. (2002). H2O2, Which Causes Macrophage-Related Stress, Triggers Induction of Expression of Virulence-Associated Plasmid Determinants in Rhodococcus equi. Infect. Immun. 70: 3768-3776 [Abstract] [Full Text]  
  • Inglis, N. F., Stevenson, K., Davies, R. C., Heaslip, D. G., Sharp, J. M. (2001). Unique expression of a highly conserved mycobacterial gene in IS901+ Mycobacterium avium. Microbiology 147: 1557-1564 [Abstract] [Full Text]  
  • Manabe, Y. C., Saviola, B. J., Sun, L., Murphy, J. R., Bishai, W. R. (1999). Attenuation of virulence in Mycobacterium tuberculosis expressing a constitutively active iron repressor. Proc. Natl. Acad. Sci. USA 96: 12844-12848 [Abstract] [Full Text]  
  • Feese, M. D., Ingason, B. P., Goranson-Siekierke, J., Holmes, R. K., Hol, W. G. J. (2001). Crystal Structure of the Iron-dependent Regulator from Mycobacterium tuberculosis at 2.0-A Resolution Reveals the Src Homology Domain 3-like Fold and Metal Binding Function of the Third Domain. J. Biol. Chem. 276: 5959-5966 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»