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Journal of Bacteriology, June 1999, p. 3427-3432, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Organization and Differential Regulation of a Cluster of Lignin Peroxidase Genes of Phanerochaete chrysosporium

Philip Stewart1,dagger and Daniel Cullen1,2,*

Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706,1 and USDA Forest Products Laboratory, Madison, Wisconsin 537052

Received 17 February 1999/Accepted 6 April 1999

The lignin peroxidases of Phanerochaete chrysosporium are encoded by a minimum of 10 closely related genes. Physical and genetic mapping of a cluster of eight lip genes revealed six genes occurring in pairs and transcriptionally convergent, suggesting that portions of the lip family arose by gene duplication events. The completed sequence of lipG and lipJ, together with previously published sequences, allowed phylogenetic and intron/exon classifications, indicating two main branches within the lip family. Competitive reverse transcription-PCR was used to assess lip transcript levels in both carbon- and nitrogen-limited media. Transcript patterns showed differential regulation of lip genes in response to medium composition. No apparent correlation was observed between genomic organization and transcript levels. Both constitutive and upregulated transcripts, structurally unrelated to peroxidases, were identified within the lip cluster.

* Corresponding author. Mailing address: USDA Forest Products Laboratory, One Gifford Pinchot Dr., Madison, WI 53705. Phone: (608) 231-9468. Fax: (608) 231-9488. E-mail: dcullen{at}facstaff.wisc.edu.

dagger Present address: Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840.

Journal of Bacteriology, June 1999, p. 3427-3432, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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