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Journal of Bacteriology, June 1999, p. 3582-3586, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning of the Glutamyl-tRNA Synthetase (gltX) Gene from Pseudomonas aeruginosa

Clifton V. Franklund and Joanna B. Goldberg*

Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908

Received 28 January 1999/Accepted 12 April 1999

The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti.

* Corresponding author. Mailing address: Department of Microbiology, University of Virginia Health Sciences Center, Box 441, Charlottesville, VA 22908. Phone: (804) 243-2774. Fax: (804) 982-1071. E-mail: jbg2b{at}virginia.edu.

Journal of Bacteriology, June 1999, p. 3582-3586, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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