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Journal of Bacteriology, June 1999, p. 3587-3590, Vol. 181, No. 11
Department of Microbiology and Molecular
Genetics, Harvard Medical School, Boston, Massachusetts 02115
Received 19 January 1999/Accepted 7 April 1999
High-level expression of the major pilus subunit (PapA) of
uropathogenic strains of Escherichia coli results in part
from the unusually long lifetime of the mRNA that encodes this protein. Here we report that the longevity of papA mRNA derives in
large measure from the protection afforded by its 5' untranslated
region. This papA RNA segment can prolong the lifetime of
an otherwise short-lived mRNA to which it is fused. In vivo alkylation
studies indicate that, in its natural milieu, the papA
message begins with a stem-loop structure. This stem-loop is important
for the stabilizing effect of the papA 5' untranslated
region, as evidenced by the significant acceleration in
papA mRNA decay that results from its removal.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Importance of a 5' Stem-Loop for Longevity of
papA mRNA in Escherichia coli
and
*
Corresponding author. Mailing address: Skirball
Institute of Biomolecular Medicine, New York University School of
Medicine, 540 First Ave., New York, NY 10016. Phone: (212) 263-5409. Fax: (212) 263-8951. E-mail:
belasco{at}saturn.med.nyu.edu.
Present address: Department of Molecular Biology and Microbiology,
Tufts University School of Medicine, Boston, MA 02111.
Journal of Bacteriology, June 1999, p. 3587-3590, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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