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Journal of Bacteriology, June 1999, p. 3591-3593, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Membrane Topology of MntB, the Transmembrane Protein Component of an ABC Transporter System for Manganese in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Victor V. Bartsevichdagger and Himadri B. Pakrasi*

Department of Biology, Washington University, St. Louis, Missouri 63130

Received 24 November 1998/Accepted 29 March 1999

The structure of the membrane protein MntB, a component of a manganese transporter system in Synechocystis sp. strain PCC 6803, was examined with a series of fusions to the reporter proteins alkaline phosphatase and beta -galactosidase. The results support a topological model for MntB consisting of nine transmembrane segments, with the amino terminus of the protein being in the periplasm and the carboxyl terminus being in the cytoplasm.

* Corresponding author. Mailing address: Department of Biology, Box 1137, Washington University, St. Louis, MO 63130. Phone: (314) 935-6853. Fax: (314) 935-6803. E-mail: Pakrasi{at}biology.wustl.edu.

dagger Present address: Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599.

Journal of Bacteriology, June 1999, p. 3591-3593, Vol. 181, No. 11
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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