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Journal of Bacteriology, June 1999, p. 3710-3715, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Morphological Transition of Helicobacter pylori Cells from Spiral to Coccoid Is Preceded by a Substantial Modification of the Cell Wall

Katyssulla Costa,1,2,3 Gerold Bacher,4 Günter Allmaier,4 María Gloria Dominguez-Bello,5 Lars Engstrand,2,3 Per Falk,6,7 Miguel A. de Pedro,1,* and Francisco García-del Portillo1

Centro de Biología Molecular "Severo Ochoa", CSIC-UAM, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain1; Institute for Analytical Chemistry, University of Vienna, A-1090 Vienna, Austria4; Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, IVIC, Caracas 1020, Venezuela5; and Swedish Institute for Infectious Disease Control, SE-17182 Solna,2 Department of Medical Epidemiology, Karolinska Institute, SE-17177 Stockholm,3 Department of Medicine, Karolinska Institute, SE-17176 Stockholm,6 and Department of Molecular Biology, ASTRA Hässle AB, SE-43183 Mölndal,7 Sweden

Received 13 January 1999/Accepted 14 April 1999

The peptidoglycan (murein) of Helicobacter pylori has been investigated by high-performance liquid chromatography and mass spectrometric techniques. Murein from H. pylori corresponded to the A1gamma chemotype, but the muropeptide elution patterns were substantially different from the one for Escherichia coli in that the former produced high proportions of muropeptides with a pentapeptide side chain (about 60 mol%), with Gly residues as the C-terminal amino acid (5 to 10 mol%), and with (1right-arrow6)anhydro-N-acetylmuramic acid (13 to 18 mol%). H. pylori murein also lacks murein-bound lipoprotein, trimeric muropeptides, and (L-D) cross-linked muropeptides. Cessation of growth and transition to coccoid shape triggered an increase in N-acetylglucosaminyl-N-acetylmuramyl-L-Ala-D-Glu (approximately 20 mol%), apparently at the expense of monomeric muropeptides with tri- and tetrapeptide side chains. Muropeptides with (1right-arrow6)anhydro-muramic acid and with Gly were also more abundant in resting cells.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa", CSIC-UAM, Facultad de Ciencias, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain. Phone: (34-91)3978083. Fax: (34-91)3978087. E-mail: madepedro{at}cbm.uam.es.

Journal of Bacteriology, June 1999, p. 3710-3715, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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