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Journal of Bacteriology, June 1999, p. 3716-3720, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Functioning of DcuC as the C4-Dicarboxylate Carrier during Glucose Fermentation by Escherichia coli

Evelyn Zientz, Ingo G. Janausch, Stephan Six, and Gottfried Unden*

Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, 55099 Mainz, Germany

Received 21 December 1998/Accepted 7 April 1999

The dcuC gene of Escherichia coli encodes an alternative C4-dicarboxylate carrier (DcuC) with low transport activity. The expression of dcuC was investigated. dcuC was expressed only under anaerobic conditions; nitrate and fumarate caused slight repression and stimulation of expression, respectively. Anaerobic induction depended mainly on the transcriptional regulator FNR. Fumarate stimulation was independent of the fumarate response regulator DcuR. The expression of dcuC was not significantly inhibited by glucose, assigning a role to DcuC during glucose fermentation. The inactivation of dcuC increased fumarate-succinate exchange and fumarate uptake by DcuA and DcuB, suggesting a preferential function of DcuC in succinate efflux during glucose fermentation. Upon overexpression in a dcuC promoter mutant (dcuC*), DcuC was able to compensate for DcuA and DcuB in fumarate-succinate exchange and fumarate uptake.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Weinforschung, Universität Mainz, Becherweg 15, 55099 Mainz, Germany. Phone: 49-6131-39/3550. Fax: 49-6131-39/2695. E-mail: unden{at}mail.uni-mainz.de.

Journal of Bacteriology, June 1999, p. 3716-3720, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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