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Journal of Bacteriology, June 1999, p. 3721-3729, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Proliferation of Intrahyphal Hyphae Caused by Disruption of csmA, Which Encodes a Class V Chitin Synthase with a Myosin Motor-Like Domain in Aspergillus nidulans

Hiroyuki Horiuchi,1,* Makoto Fujiwara,1,dagger Shuichi Yamashita,2 Akinori Ohta,1 and Masamichi Takagi1

Department of Biotechnology1 and Department of Agricultural and Environmental Biology,2 The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan

Received 29 January 1999/Accepted 15 March 1999

We have found that the Aspergillus nidulans csmA gene encodes a novel protein which consists of an N-terminal myosin motor-like domain and a C-terminal chitin synthase domain (M. Fujiwara, H. Horiuchi, A. Ohta, and M. Takagi, Biochem. Biophys. Res. Commun. 236:75-78, 1997). To clarify the roles of csmA in fungal morphogenesis, we constructed csmA null mutants. The growth rate of the mutant colonies was almost the same as that of the wild-type strain, but hyphal growth was severely inhibited when a chitin-binding reagent, Calcofluor white or Congo red, was added to the medium. Moreover, morphological abnormalities in tip growth and septum formation were identified microscopically. Proliferation of intracellular new hyphae, called intrahyphal hyphae, which behaved as intrinsic hyphae, was the most striking phenotypic feature among them. These phenotypes were not suppressed when the only chitin synthase domain of csmA was expressed under the control of the alcA promoter, whereas they were suppressed when the intact form of csmA was expressed. Therefore, it was concluded that the product of csmA (CsmA) has important roles in polarized cell wall synthesis and maintenance of cell wall integrity and that the myosin motor-like domain is indispensable for these functions.

* Corresponding author. Mailing address: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone and fax: 81-3-5841-8015. E-mail: amtakag{at}hongo.ecc.u-tokyo.ac.jp.

dagger Present address: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-8657, Japan.

Journal of Bacteriology, June 1999, p. 3721-3729, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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