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Journal of Bacteriology, June 1999, p. 3768-3776, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Transcriptional Organization and In Vivo Role of the
Escherichia coli rsd Gene, Encoding the Regulator of RNA
Polymerase Sigma D
Miki
Jishage1,2 and
Akira
Ishihama1,*
Department of Molecular Genetics, National
Institute of Genetics, Mishima, Shizuoka
411-8540,1 and Japan Science and
Technology Corporation, Kawaguchi, Saitama
332-0012,2 Japan
Received 11 February 1999/Accepted 21 April 1999
The regulator of sigma D (Rsd) was identified as an RNA polymerase
70-associated protein in stationary-phase
Escherichia coli with the inhibitory activity of
70-dependent transcription in vitro (M. Jishage and
A. Ishihama, Proc. Natl. Acad. Sci. USA 95:4953-4958, 1998).
Primer extension analysis of rsd mRNA indicated the
presence of two promoters,
S-dependent P1 and
70-dependent P2 with the gearbox sequence. To get
insight into the in vivo role of Rsd, the expression of a reporter gene
fused to either the
70- or
S-dependent
promoter was analyzed in the absence of Rsd or the presence of
overexpressed Rsd. In the rsd null mutant, the
70- and
S-dependent gene expression was
increased or decreased, respectively. On the other hand, the
70- or
S-dependent transcription was
reduced or enhanced, respectively, after overexpression of Rsd.
The repression of the
S-dependent transcription in
the rsd mutant is overcome by increased production of the
S subunit. Together these observations support the
prediction that Rsd is involved in replacement of the RNA polymerase
subunit from
70 to
S during the
transition from exponential growth to the stationary phase.
*
Corresponding author. Mailing address: Department of
Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. Phone: 81-559-81-6741. Fax: 81-559-81-6746. E-mail: aishiham{at}lab.nig.ac.jp.
Journal of Bacteriology, June 1999, p. 3768-3776, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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