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Journal of Bacteriology, June 1999, p. 3768-3776, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transcriptional Organization and In Vivo Role of the Escherichia coli rsd Gene, Encoding the Regulator of RNA Polymerase Sigma D

Miki Jishage1,2 and Akira Ishihama1,*

Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540,1 and Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012,2 Japan

Received 11 February 1999/Accepted 21 April 1999

The regulator of sigma D (Rsd) was identified as an RNA polymerase sigma 70-associated protein in stationary-phase Escherichia coli with the inhibitory activity of sigma 70-dependent transcription in vitro (M. Jishage and A. Ishihama, Proc. Natl. Acad. Sci. USA 95:4953-4958, 1998). Primer extension analysis of rsd mRNA indicated the presence of two promoters, sigma S-dependent P1 and sigma 70-dependent P2 with the gearbox sequence. To get insight into the in vivo role of Rsd, the expression of a reporter gene fused to either the sigma 70- or sigma S-dependent promoter was analyzed in the absence of Rsd or the presence of overexpressed Rsd. In the rsd null mutant, the sigma 70- and sigma S-dependent gene expression was increased or decreased, respectively. On the other hand, the sigma 70- or sigma S-dependent transcription was reduced or enhanced, respectively, after overexpression of Rsd. The repression of the sigma S-dependent transcription in the rsd mutant is overcome by increased production of the sigma S subunit. Together these observations support the prediction that Rsd is involved in replacement of the RNA polymerase sigma  subunit from sigma 70 to sigma S during the transition from exponential growth to the stationary phase.

* Corresponding author. Mailing address: Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. Phone: 81-559-81-6741. Fax: 81-559-81-6746. E-mail: aishiham{at}lab.nig.ac.jp.

Journal of Bacteriology, June 1999, p. 3768-3776, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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