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Journal of Bacteriology, June 1999, p. 3777-3783, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transcriptional Regulation in the Hyperthermophilic Archaeon Pyrococcus furiosus: Coordinated Expression of Divergently Oriented Genes in Response to beta -Linked Glucose Polymers

Wilfried G. B. Voorhorst,1 Yannick Gueguen,1 Ans C. M. Geerling,1 Gerti Schut,1 Isabell Dahlke,2 Michael Thomm,2 John van der Oost,1,* and Willem M. de Vos1

Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, NL-6703 CT Wageningen, The Netherlands,1 and Institut für Allgemeine Mikrobiologie der Christian-Albrechts-Universität zu Kiel, D-24118 Kiel, Germany2

Received 12 February 1999/Accepted 19 April 1999

The genetic organization, expression, and regulation of the celB locus of the hyperthermophilic archaeon Pyrococcus furiosus were analyzed. This locus includes the celB gene, which codes for an intracellular beta -glucosidase, and a divergently orientated gene cluster, adhA-adhB-lamA, which codes for two alcohol dehydrogenases and an extracellular beta -1,3-endoglucanase that is transcribed as a polycistronic messenger (the lamA operon). During growth of P. furiosus on either the beta -1,4-linked glucose dimer cellobiose or the beta -1,3-linked glucose polymer laminarin, the activities of both beta -glucosidase and endoglucanase were increased at least fivefold compared with levels during growth on maltose or pyruvate. Northern blot analysis revealed an enhanced transcription of both the celB gene and the lamA operon in the presence of these glucose-containing substrates. The in vivo and in vitro transcription initiation sites of both the celB gene and the lamA operon were identified 25 nucleotides downstream of conserved TATA box motifs. A number of repeating sequences have been recognized in the celB-adhA intergenic region, some of which might be part of a transcriptional regulator-binding site.

* Corresponding author. Mailing address: Laboratory of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Phone: 31 317 483100. Fax: 31 317 483829. E-mail: john.vanderoost{at}algemeen.micr.wau.nl.

Journal of Bacteriology, June 1999, p. 3777-3783, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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