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Journal of Bacteriology, June 1999, p. 3837-3841, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Biochemical and Molecular Characterization of the
Bacillus subtilis Acetoin Catabolic Pathway
Min
Huang,
Fred Bernd
Oppermann-Sanio, and
Alexander
Steinbüchel*
Institut für Mikrobiologie der
Westfälischen Wilhelms-Universität Münster, D-48149
Münster, Germany
Received 2 October 1998/Accepted 5 April 1999
A recent study indicated that Bacillus subtilis
catabolizes acetoin by enzymes encoded by the acu gene
cluster (F. J. Grundy, D. A. Waters, T. Y. Takova, and
T. M. Henkin, Mol. Microbiol. 10:259-271, 1993) that are
completely different from those in the multicomponent acetoin
dehydrogenase enzyme system (AoDH ES) encoded by aco gene
clusters found before in all other bacteria capable of utilizing
acetoin as the sole carbon source for growth. By hybridization with a
DNA probe covering acoA and acoB of the AoDH ES
from Clostridium magnum, genomic fragments from B. subtilis harboring acoA, acoB,
acoC, acoL, and acoR homologous
genes were identified, and some of them were functionally expressed in
E. coli. Furthermore, acoA was inactivated in
B. subtilis by disruptive mutagenesis; these mutants were
impaired to express PPi-dependent AoDH E1 activity to
remove acetoin from the medium and to grow with acetoin as the carbon
source. Therefore, acetoin is catabolized in B. subtilis by
the same mechanism as all other bacteria investigated so far, leaving
the function of the previously described acu genes obscure.
*
Corresponding author. Mailing address: Institut
für Mikrobiologie der Westfälischen
Wilhelms-Universität Münster, Corrensstraße 3, Germany.
Phone: 49-251-8339821. Fax: 49-251-8338388. E-mail: steinbu{at}uni-muenster.de.
Journal of Bacteriology, June 1999, p. 3837-3841, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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