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Journal of Bacteriology, June 1999, p. 3845-3848, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Characterization of the Gene Encoding Pasteurella haemolytica FnrP, a Regulator of the Escherichia coli Silent Hemolysin SheAdagger

Gaylen A. Uhlich,1,Dagger Peter J. McNamara,2 John J. Iandolo,3 and Derek A. Mosier1,*

Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 665061; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine, Madison, Wisconsin 537062; and Department of Microbiology and Immunology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 739013

Received 19 October 1998/Accepted 12 April 1999

A Pasteurella haemolytica A1 gene was identified from a recombinant library clone that expressed hemolysis in host Escherichia coli cells. The gene, designated fnrP, had sequence identity to E. coli fnr, a global transcriptional regulator of genes required for conversion to anaerobic growth. FnrP complemented anaerobic deficiencies of a fnr-null mutant strain of E. coli and increased expression of the Fnr-dependent, anaerobic terminal reductase gene, frdA. FnrP was purified, identified by immunoblotting, and shown to be nonhemolytic. When FnrP was expressed in E. coli Delta sheA, a null mutant of the cryptic hemolysin SheA, the transformants were nonhemolytic, indicating that FnrP activates this silent hemolysin.

* Corresponding author. Mailing address: Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4410. Fax: (785) 532-4039. E-mail: mosier_d{at}vet.ksu.edu.

dagger Contribution 99-409-J of the Kansas Agricultural Experiment Station.

Dagger Present address: Roman L. Hruska U.S. Meat Animal Research Center, ARS, USDA, P.O. Box 166, Clay Center, NE 68933.

Journal of Bacteriology, June 1999, p. 3845-3848, Vol. 181, No. 12
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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