Amino Acid Residues in the omega -Minus Region Participate in Cellular Localization of Yeast Glycosylphosphatidylinositol-Attached Proteins

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Journal of Bacteriology, July 1999, p. 3886-3889, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Amino Acid Residues in the $omega$ -Minus Region Participate in Cellular Localization of Yeast Glycosylphosphatidylinositol-Attached Proteins

Kenji Hamada, Hiromichi Terashima, Mikio Arisawa, Nami Yabuki, and Kunio Kitada^*

Department of Mycology, Nippon Roche Research Center, Kamakura, Kanagawa 247-8530, Japan

Received 10 March 1999/Accepted 29 April 1999

The final destination of glycosylphosphatidylinositol (GPI)-attached proteins in Saccharomyces cerevisiae is the plasma membraneor the cell wall. Two kinds of signals have been proposed fortheir cellular localization: (i) the specific amino acid residuesV, I, or L at the site 4 or 5 amino acids upstream of the GPIattachment site (the $omega$ site) and Y or N at the site 2 amino acidsupstream of the $omega$ site for cell wall localization and (ii) dibasicresidues in the region upstream of the $omega$ site (the $omega$ -minus region)for plasma membrane localization. The relationships between theseamino acid residues and efficiencies of cell wall incorporationwere examined by constructing fusion reporter proteins from openreading frames encoding putative GPI-attached proteins. The levelsof incorporation were high in the constructs containing the specificamino acid residues and quite low in those containing two basicamino acid residues in the $omega$ -minus region. With constructs thatcontained neither specific residues nor two basic residues, levelsof incorporation were moderate. These correlations clearly suggestthat GPI-attached proteins have two different signals which actpositively or negatively in cell wall incorporation for theircellularlocalization.

^* Corresponding author. Mailing address: Department of Mycology, Nippon Roche Research Center, 200-Kajiwara, Kamakura, Kanagawa247-8530, Japan. Phone: 81-467-45-6752. Fax: 81-467-46-5320. E-mail:kunio.kitada{at}roche.com.

Journal of Bacteriology, July 1999, p. 3886-3889, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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Amino Acid Residues in the -Minus Region Participate in Cellular Localization of Yeast Glycosylphosphatidylinositol-Attached Proteins

Amino Acid Residues in the $omega$ -Minus Region Participate in Cellular Localization of Yeast Glycosylphosphatidylinositol-Attached Proteins