This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suh, S.-J.
Right arrow Articles by Ohman, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suh, S.-J.
Right arrow Articles by Ohman, D. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 1999, p. 3890-3897, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Effect of rpoS Mutation on the Stress Response and Expression of Virulence Factors in Pseudomonas aeruginosa

Sang-Jin Suh,1,2,* Laura Silo-Suh,2 Donald E. Woods,3 Daniel J. Hassett,4 Susan E. H. West,5 and Dennis E. Ohman2

Department of Microbiology and Immunology, University of Tennessee, and Veterans Affairs Medical Center, Memphis, Tennessee 381041; Department of Microbiology and Immunology, Medical College of Virginia campus of Virginia Commonwealth University, and McGuire Veterans Affairs Medical Center, Richmond, Virginia 23298-06782; Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada T2N 4N13; Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 452574; and Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin---Madison, Madison, Wisconsin 537065

Received 7 December 1998/Accepted 20 April 1999

The sigma factor RpoS (sigma S) has been described as a general stress response regulator that controls the expression of genes which confer increased resistance to various stresses in some gram-negative bacteria. To elucidate the role of RpoS in Pseudomonas aeruginosa physiology and pathogenesis, we constructed rpoS mutants in several strains of P. aeruginosa, including PAO1. The PAO1 rpoS mutant was subjected to various environmental stresses, and we compared the resistance phenotype of the mutant to that of the parent. The PAO1 rpoS mutant was slightly more sensitive to carbon starvation than the wild-type strain, but this phenotype was obvious only when the cells were grown in a medium supplemented with glucose as the sole carbon source. In addition, the PAO1 rpoS mutant was hypersensitive to heat shock at 50°C, increased osmolarity, and prolonged exposure to high concentrations of H2O2. In accordance with the hypersensitivity to H2O2, catalase production was 60% lower in the rpoS mutant than in the parent strain. We also assessed the role of RpoS in the production of several exoproducts known to be important for virulence of P. aeruginosa. The rpoS mutant produced 50% less exotoxin A, but it produced only slightly smaller amounts of elastase and LasA protease than the parent strain. The levels of phospholipase C and casein-degrading proteases were unaffected by a mutation in rpoS in PAO1. The rpoS mutation resulted in the increased production of the phenazine antibiotic pyocyanin and the siderophore pyoverdine. This increased pyocyanin production may be responsible for the enhanced virulence of the PAO1 rpoS mutant that was observed in a rat chronic-lung-infection model. In addition, the rpoS mutant displayed an altered twitching-motility phenotype, suggesting that the colonization factors, type IV fimbriae, were affected. Finally, in an alginate-overproducing cystic fibrosis (CF) isolate, FRD1, the rpoS101::aacCI mutation almost completely abolished the production of alginate when the bacterium was grown in a liquid medium. On a solid medium, the FRD1 rpoS mutant produced approximately 70% less alginate than did the wild-type strain. Thus, our data indicate that although some of the functions of RpoS in P. aeruginosa physiology are similar to RpoS functions in other gram-negative bacteria, it also has some functions unique to this bacterium.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Virginia campus of Virginia Commonwealth University, 1101 East Marshall St., P.O. Box 980678, Richmond, VA 23298-0678. Phone: (804) 628-0247. Fax: (804) 828-9946. E-mail: ssuh{at}hsc.vcu.edu.

Journal of Bacteriology, July 1999, p. 3890-3897, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hagins, J. M., Locy, R., Silo-Suh, L. (2009). Isocitrate Lyase Supplies Precursors for Hydrogen Cyanide Production in a Cystic Fibrosis Isolate of Pseudomonas aeruginosa. J. Bacteriol. 191: 6335-6339 [Abstract] [Full Text]  
  • Hovel-Miner, G., Pampou, S., Faucher, S. P., Clarke, M., Morozova, I., Morozov, P., Russo, J. J., Shuman, H. A., Kalachikov, S. (2009). {sigma}S Controls Multiple Pathways Associated with Intracellular Multiplication of Legionella pneumophila. J. Bacteriol. 191: 2461-2473 [Abstract] [Full Text]  
  • Dong, T., Coombes, B. K., Schellhorn, H. E. (2009). Role of RpoS in the Virulence of Citrobacter rodentium. Infect. Immun. 77: 501-507 [Abstract] [Full Text]  
  • Manos, J., Arthur, J., Rose, B., Tingpej, P., Fung, C., Curtis, M., Webb, J. S., Hu, H., Kjelleberg, S., Gorrell, M. D., Bye, P., Harbour, C. (2008). Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung. J Med Microbiol 57: 1454-1465 [Abstract] [Full Text]  
  • Weir, T. L., Stull, V. J., Badri, D., Trunck, L. A., Schweizer, H. P., Vivanco, J. (2008). Global Gene Expression Profiles Suggest an Important Role for Nutrient Acquisition in Early Pathogenesis in a Plant Model of Pseudomonas aeruginosa Infection. Appl. Environ. Microbiol. 74: 5784-5791 [Abstract] [Full Text]  
  • Lindsey, T. L., Hagins, J. M., Sokol, P. A., Silo-Suh, L. A. (2008). Virulence determinants from a cystic fibrosis isolate of Pseudomonas aeruginosa include isocitrate lyase. Microbiology 154: 1616-1627 [Abstract] [Full Text]  
  • Hughes, J. L., Nolder, C. L., Nowalk, A. J., Clifton, D. R., Howison, R. R., Schmit, V. L., Gilmore, R. D. Jr., Carroll, J. A. (2008). Borrelia burgdorferi Surface-Localized Proteins Expressed during Persistent Murine Infection Are Conserved among Diverse Borrelia spp.. Infect. Immun. 76: 2498-2511 [Abstract] [Full Text]  
  • Goldsworthy, M. J. H. (2008). Gene expression of Pseudomonas aeruginosa and MRSA within a catheter-associated urinary tract infection biofilm model. Bioscience Horizons 1: 28-37 [Abstract] [Full Text]  
  • Sandercock, J. R., Page, W. J. (2008). RpoS Expression and the General Stress Response in Azotobacter vinelandii during Carbon and Nitrogen Diauxic Shifts. J. Bacteriol. 190: 946-953 [Abstract] [Full Text]  
  • Schaber, J. A., Triffo, W. J., Suh, S. J., Oliver, J. W., Hastert, M. C., Griswold, J. A., Auer, M., Hamood, A. N., Rumbaugh, K. P. (2007). Pseudomonas aeruginosa Forms Biofilms in Acute Infection Independent of Cell-to-Cell Signaling. Infect. Immun. 75: 3715-3721 [Abstract] [Full Text]  
  • Muller, J. F., Stevens, A. M., Craig, J., Love, N. G. (2007). Transcriptome Analysis Reveals that Multidrug Efflux Genes Are Upregulated To Protect Pseudomonas aeruginosa from Pentachlorophenol Stress. Appl. Environ. Microbiol. 73: 4550-4558 [Abstract] [Full Text]  
  • Chatterjee, A., Cui, Y., Hasegawa, H., Chatterjee, A. K. (2007). PsrA, the Pseudomonas Sigma Regulator, Controls Regulators of Epiphytic Fitness, Quorum-Sensing Signals, and Plant Interactions in Pseudomonas syringae pv. tomato Strain DC3000. Appl. Environ. Microbiol. 73: 3684-3694 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Nordmann, P. (2006). Regulation of class D beta-lactamase gene expression in Ralstonia pickettii.. Microbiology 152: 2661-2672 [Abstract] [Full Text]  
  • Girard, G., van Rij, E. T., Lugtenberg, B. J. J., Bloemberg, G. V. (2006). Regulatory roles of psrA and rpoS in phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis PCL1391. Microbiology 152: 43-58 [Abstract] [Full Text]  
  • Kazmierczak, M. J., Wiedmann, M., Boor, K. J. (2005). Alternative Sigma Factors and Their Roles in Bacterial Virulence. Microbiol. Mol. Biol. Rev. 69: 527-543 [Abstract] [Full Text]  
  • Silo-Suh, L., Suh, S.-J., Phibbs, P. V., Ohman, D. E. (2005). Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase. J. Bacteriol. 187: 7561-7568 [Abstract] [Full Text]  
  • Stockwell, V. O., Loper, J. E. (2005). The sigma factor RpoS is required for stress tolerance and environmental fitness of Pseudomonas fluorescens Pf-5. Microbiology 151: 3001-3009 [Abstract] [Full Text]  
  • Kosono, S., Haga, K., Tomizawa, R., Kajiyama, Y., Hatano, K., Takeda, S., Wakai, Y., Hino, M., Kudo, T. (2005). Characterization of a Multigene-Encoded Sodium/Hydrogen Antiporter (Sha) from Pseudomonas aeruginosa: Its Involvement in Pathogenesis. J. Bacteriol. 187: 5242-5248 [Abstract] [Full Text]  
  • Parvatiyar, K., Alsabbagh, E. M., Ochsner, U. A., Stegemeyer, M. A., Smulian, A. G., Hwang, S. H., Jackson, C. R., McDermott, T. R., Hassett, D. J. (2005). Global Analysis of Cellular Factors and Responses Involved in Pseudomonas aeruginosa Resistance to Arsenite. J. Bacteriol. 187: 4853-4864 [Abstract] [Full Text]  
  • Lee, J.-S., Heo, Y.-J., Lee, J. K., Cho, Y.-H. (2005). KatA, the Major Catalase, Is Critical for Osmoprotection and Virulence in Pseudomonas aeruginosa PA14. Infect. Immun. 73: 4399-4403 [Abstract] [Full Text]  
  • Caimano, M. J., Eggers, C. H., Hazlett, K. R. O., Radolf, J. D. (2004). RpoS Is Not Central to the General Stress Response in Borrelia burgdorferi but Does Control Expression of One or More Essential Virulence Determinants. Infect. Immun. 72: 6433-6445 [Abstract] [Full Text]  
  • Erickson, D. L., Lines, J. L., Pesci, E. C., Venturi, V., Storey, D. G. (2004). Pseudomonas aeruginosa relA Contributes to Virulence in Drosophila melanogaster. Infect. Immun. 72: 5638-5645 [Abstract] [Full Text]  
  • Lizewski, S. E., Schurr, J. R., Jackson, D. W., Frisk, A., Carterson, A. J., Schurr, M. J. (2004). Identification of AlgR-Regulated Genes in Pseudomonas aeruginosa by Use of Microarray Analysis. J. Bacteriol. 186: 5672-5684 [Abstract] [Full Text]  
  • Sevo, M., Buratti, E., Venturi, V. (2004). Ribosomal Protein S1 Specifically Binds to the 5' Untranslated Region of the Pseudomonas aeruginosa Stationary-Phase Sigma Factor rpoS mRNA in the Logarithmic Phase of Growth. J. Bacteriol. 186: 4903-4909 [Abstract] [Full Text]  
  • Park, K.-J., Kang, M.-J., Kim, S. H., Lee, H.-J., Lim, J.-K., Choi, S. H., Park, S.-J., Lee, K.-H. (2004). Isolation and Characterization of rpoS from a Pathogenic Bacterium, Vibrio vulnificus: Role of {sigma}S in Survival of Exponential-Phase Cells under Oxidative Stress. J. Bacteriol. 186: 3304-3312 [Abstract] [Full Text]  
  • Hogardt, M., Roeder, M., Schreff, A. M., Eberl, L., Heesemann, J. (2004). Expression of Pseudomonas aeruginosa exoS is controlled by quorum sensing and RpoS. Microbiology 150: 843-851 [Abstract] [Full Text]  
  • Ramos-Aires, J., Plesiat, P., Kocjancic-Curty, L., Kohler, T. (2004). Selection of an Antibiotic-Hypersusceptible Mutant of Pseudomonas aeruginosa: Identification of the GlmR Transcriptional Regulator. Antimicrob. Agents Chemother. 48: 843-851 [Abstract] [Full Text]  
  • Subsin, B., Thomas, M. S., Katzenmeier, G., Shaw, J. G., Tungpradabkul, S., Kunakorn, M. (2003). Role of the Stationary Growth Phase Sigma Factor RpoS of Burkholderia pseudomallei in Response to Physiological Stress Conditions. J. Bacteriol. 185: 7008-7014 [Abstract] [Full Text]  
  • Chen, G., Patten, C. L., Schellhorn, H. E. (2003). Controlled Expression of an rpoS Antisense RNA Can Inhibit RpoS Function in Escherichia coli. Antimicrob. Agents Chemother. 47: 3485-3493 [Abstract] [Full Text]  
  • Hulsmann, A., Rosche, T. M., Kong, I.-S., Hassan, H. M., Beam, D. M., Oliver, J. D. (2003). RpoS-Dependent Stress Response and Exoenzyme Production in Vibrio vulnificus. Appl. Environ. Microbiol. 69: 6114-6120 [Abstract] [Full Text]  
  • Kazmierczak, M. J., Mithoe, S. C., Boor, K. J., Wiedmann, M. (2003). Listeria monocytogenes {sigma}B Regulates Stress Response and Virulence Functions. J. Bacteriol. 185: 5722-5734 [Abstract] [Full Text]  
  • Opdyke, J. A., Scott, J. R., Moran, C. P. Jr. (2003). Expression of the Secondary Sigma Factor {sigma}X in Streptococcus pyogenes Is Restricted at Two Levels. J. Bacteriol. 185: 4291-4297 [Abstract] [Full Text]  
  • O'Toole, G. A. (2003). To Build a Biofilm. J. Bacteriol. 185: 2687-2689 [Full Text]  
  • Cooper, M., Tavankar, G. R., Williams, H. D. (2003). Regulation of expression of the cyanide-insensitive terminal oxidase in Pseudomonas aeruginosa. Microbiology 149: 1275-1284 [Abstract] [Full Text]  
  • Aguilar, C., Bertani, I., Venturi, V. (2003). Quorum-Sensing System and Stationary-Phase Sigma Factor (rpoS) of the Onion Pathogen Burkholderia cepacia Genomovar I Type Strain, ATCC 25416. Appl. Environ. Microbiol. 69: 1739-1747 [Abstract] [Full Text]  
  • Medina, G., Juarez, K., Soberon-Chavez, G. (2003). The Pseudomonas aeruginosa rhlAB Operon Is Not Expressed during the Logarithmic Phase of Growth Even in the Presence of Its Activator RhlR and the Autoinducer N-Butyryl-Homoserine Lactone. J. Bacteriol. 185: 377-380 [Abstract] [Full Text]  
  • Silo-Suh, L., Suh, S.-J., Sokol, P. A., Ohman, D. E. (2002). A simple alfalfa seedling infection model for Pseudomonas aeruginosa strains associated with cystic fibrosis shows AlgT (sigma-22) and RhlR contribute to pathogenesis. Proc. Natl. Acad. Sci. USA 99: 15699-15704 [Abstract] [Full Text]  
  • Hengge-Aronis, R. (2002). Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase. Microbiol. Mol. Biol. Rev. 66: 373-395 [Abstract] [Full Text]  
  • Laux, D. C., Corson, J. M., Givskov, M., Hentzer, M., Moller, A., Wosencroft, K. A., Olson, J. C., Krogfelt, K. A., Goldberg, J. B., Cohen, P. S. (2002). Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and LasA. Microbiology 148: 1709-1723 [Abstract] [Full Text]  
  • Diggle, S. P., Winzer, K., Lazdunski, A., Williams, P., Camara, M. (2002). Advancing the Quorum in Pseudomonas aeruginosa: MvaT and the Regulation of N-Acylhomoserine Lactone Production and Virulence Gene Expression. J. Bacteriol. 184: 2576-2586 [Abstract] [Full Text]  
  • Kojic, M., Aguilar, C., Venturi, V. (2002). TetR Family Member PsrA Directly Binds the Pseudomonas rpoS and psrA Promoters. J. Bacteriol. 184: 2324-2330 [Abstract] [Full Text]  
  • Heydorn, A., Ersboll, B., Kato, J., Hentzer, M., Parsek, M. R., Tolker-Nielsen, T., Givskov, M., Molin, S. (2002). Statistical Analysis of Pseudomonas aeruginosa Biofilm Development: Impact of Mutations in Genes Involved in Twitching Motility, Cell-to-Cell Signaling, and Stationary-Phase Sigma Factor Expression. Appl. Environ. Microbiol. 68: 2008-2017 [Abstract] [Full Text]  
  • Wilson, J W, Schurr, M J, LeBlanc, C L, Ramamurthy, R, Buchanan, K L, Nickerson, C A (2002). Mechanisms of bacterial pathogenicity. Postgrad. Med. J. 78: 216-224 [Abstract] [Full Text]  
  • Sonnleitner, E., Moll, I., Blasi, U. (2002). Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa. Microbiology 148: 883-891 [Abstract] [Full Text]  
  • Schnider-Keel, U., Lejbolle, K. B., Baehler, E., Haas, D., Keel, C. (2001). The Sigma Factor AlgU (AlgT) Controls Exopolysaccharide Production and Tolerance towards Desiccation and Osmotic Stress in the Biocontrol Agent Pseudomonas fluorescens CHA0. Appl. Environ. Microbiol. 67: 5683-5693 [Abstract] [Full Text]  
  • Castaneda, M., Sanchez, J., Moreno, S., Nunez, C., Espin, G. (2001). The Global Regulators GacA and sigma S Form Part of a Cascade That Controls Alginate Production in Azotobacter vinelandii. J. Bacteriol. 183: 6787-6793 [Abstract] [Full Text]  
  • Pessi, G., Williams, F., Hindle, Z., Heurlier, K., Holden, M. T. G., Camara, M., Haas, D., Williams, P. (2001). The Global Posttranscriptional Regulator RsmA Modulates Production of Virulence Determinants and N-Acylhomoserine Lactones in Pseudomonas aeruginosa. J. Bacteriol. 183: 6676-6683 [Abstract] [Full Text]  
  • Mavrodi, D. V., Bonsall, R. F., Delaney, S. M., Soule, M. J., Phillips, G., Thomashow, L. S. (2001). Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO1. J. Bacteriol. 183: 6454-6465 [Abstract] [Full Text]  
  • van Delden, C., Comte, R., Bally, A. M. (2001). Stringent Response Activates Quorum Sensing and Modulates Cell Density-Dependent Gene Expression in Pseudomonas aeruginosa. J. Bacteriol. 183: 5376-5384 [Abstract] [Full Text]  
  • Mogull, S. A., Runyen-Janecky, L. J., Hong, M., Payne, S. M. (2001). dksA Is Required for Intercellular Spread of Shigella flexneri via an RpoS-Independent Mechanism. Infect. Immun. 69: 5742-5751 [Abstract] [Full Text]  
  • Kojic, M., Venturi, V. (2001). Regulation of rpoS Gene Expression in Pseudomonas: Involvement of a TetR Family Regulator. J. Bacteriol. 183: 3712-3720 [Abstract] [Full Text]  
  • Kelly, A. F., Park, S. F., Bovill, R., Mackey, B. M. (2001). Survival of Campylobacter jejuni during Stationary Phase: Evidence for the Absence of a Phenotypic Stationary-Phase Response. Appl. Environ. Microbiol. 67: 2248-2254 [Abstract] [Full Text]  
  • Branny, P., Pearson, J. P., Pesci, E. C., Köhler, T., Iglewski, B. H., Van Delden, C. (2001). Inhibition of Quorum Sensing by a Pseudomonas aeruginosa dksA Homologue. J. Bacteriol. 183: 1531-1539 [Abstract] [Full Text]  
  • Déziel, E., Comeau, Y., Villemur, R. (2001). Initiation of Biofilm Formation by Pseudomonas aeruginosa 57RP Correlates with Emergence of Hyperpiliated and Highly Adherent Phenotypic Variants Deficient in Swimming, Swarming, and Twitching Motilities. J. Bacteriol. 183: 1195-1204 [Abstract] [Full Text]  
  • Page, W. J., Tindale, A., Chandra, M., Kwon, E. (2001). Alginate formation in Azotobacter vinelandii UWD during stationary phase and the turnover of poly-{beta}-hydroxybutyrate. Microbiology 147: 483-490 [Abstract] [Full Text]  
  • Malhotra, S., Silo-Suh, L. A., Mathee, K., Ohman, D. E. (2000). Proteome Analysis of the Effect of Mucoid Conversion on Global Protein Expression in Pseudomonas aeruginosa Strain PAO1 Shows Induction of the Disulfide Bond Isomerase, DsbA. J. Bacteriol. 182: 6999-7006 [Abstract] [Full Text]  
  • Winzer, K., Falconer, C., Garber, N. C., Diggle, S. P., Camara, M., Williams, P. (2000). The Pseudomonas aeruginosa Lectins PA-IL and PA-IIL Are Controlled by Quorum Sensing and by RpoS. J. Bacteriol. 182: 6401-6411 [Abstract] [Full Text]  
  • Heydorn, A., Ersbøll, B. K., Hentzer, M., Parsek, M. R., Givskov, M., Molin, S. (2000). Experimental reproducibility in flow-chamber biofilms. Microbiology 146: 2409-2415 [Abstract] [Full Text]  
  • Whiteley, M., Parsek, M. R., Greenberg, E. P. (2000). Regulation of Quorum Sensing by RpoS in Pseudomonas aeruginosa. J. Bacteriol. 182: 4356-4360 [Abstract] [Full Text]  
  • Weyand, N. J., Low, D. A. (2000). Regulation of Pap Phase Variation. Lrp IS SUFFICIENT FOR THE ESTABLISHMENT OF THE PHASE OFF pap DNA METHYLATION PATTERN AND REPRESSION OF pap TRANSCRIPTION IN VITRO. J. Biol. Chem. 275: 3192-3200 [Abstract] [Full Text]  
  • Rashid, M. H., Kornberg, A. (2000). Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 97: 4885-4890 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»