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Journal of Bacteriology, July 1999, p. 3898-3903, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Characterization of the Major Superoxide Dismutase
of Staphylococcus aureus and Its Role in Starvation
Survival, Stress Resistance, and Pathogenicity
Mark O.
Clements,
Sean P.
Watson, and
Simon J.
Foster*
Department of Molecular Biology and
Biotechnology, University of Sheffield, Western Bank, Sheffield S10
2TN, United Kingdom
Received 28 January 1999/Accepted 7 April 1999
A Staphylococcus aureus mutant (SPW1) which is unable
to survive long-term starvation was shown to have a transposon
insertion within a gene homologous to the sodA family of
manganese-dependent superoxide dismutases (SOD). Whole-cell lysates of
the parental 8325-4 strain demonstrated three zones of SOD activity by
nondenaturing gel electrophoresis. The activities of two of these zones
were dependent on manganese for activity and were absent in SPW1. The levels of SOD activity and sodA expression were
growth-phase dependent, occurring most during postexponential phase.
This response was also dependent on the level of aeration of the
culture, with highest activity and expression occurring only under high
aeration. Expression of sodA and, consequently, SOD
activity could be induced by methyl viologen but only during the
transition from exponential- to postexponential-phase growth. SPW1 was
less able to survive amino acid limitation and acid stress but
showed no alteration in pathogenicity in a mouse abscess model of
infection compared to the parental strain 8325-4.
*
Corresponding author. Mailing address: Department of
Molecular Biology and Biotechnology, University of Sheffield, Firth
Court, Western Bank, Sheffield S10 2TN, United Kingdom. Phone: 44 114 2224411. Fax: 44 114 2728697. E-mail:
s.foster{at}sheffield.ac.uk.
Journal of Bacteriology, July 1999, p. 3898-3903, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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