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Journal of Bacteriology, July 1999, p. 3967-3973, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Regulation of podJ Expression during the Caulobacter crescentus Cell Cycle

William B. Crymes Jr., Daolong Zhang,dagger and Bert Ely*

Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208

Received 6 October 1998/Accepted 22 April 1999

The polar organelle development gene, podJ, is expressed during the swarmer-to-stalked cell transition of the Caulobacter crescentus cell cycle. Mutants with insertions that inactivate the podJ gene are nonchemotactic, deficient in rosette formation, and resistant to polar bacteriophage, but they divide normally. In contrast, hyperexpression of podJ results in a lethal cell division defect. Nucleotide sequence analysis of the podJ promoter region revealed a binding site for the global response regulator, CtrA. Deletion of this site results in increased overall promoter activity, suggesting that CtrA is a negative regulator of the podJ promoter. Furthermore, synchronization studies have indicated that temporal regulation is not dependent on the presence of the CtrA binding site. Thus, although the level of podJ promoter activity is dependent on the CtrA binding site, the temporal control of podJ promoter expression is dependent on other factors.

* Corresponding author. Mailing address: Department of Biological Sciences, University of South Carolina, Columbia, SC 29208. Phone: (803) 777-2768. Fax: (803) 777-4002. E-mail address: ely{at}sc.edu.

dagger Present address: University of Chicago, Department of Psychiatry, Chicago, IL 60637.

Journal of Bacteriology, July 1999, p. 3967-3973, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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