This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cope, L. D.
Right arrow Articles by Hansen, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cope, L. D.
Right arrow Articles by Hansen, E. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 1999, p. 4026-4034, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of the Moraxella catarrhalis uspA1 and uspA2 Genes and Their Encoded Products

Leslie D. Cope,1 Eric R. Lafontaine,1 Clive A. Slaughter,2 Charles A. Hasemann Jr.,3 Christoph Aebi,1,4 Frederick W. Henderson,5 George H. McCracken Jr.,4 and Eric J. Hansen1,*

Departments of Microbiology,1 Biochemistry,2 Internal Medicine,3 and Pediatrics,4 University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048, and Department of Pediatrics, University of North Carolina, Chapel Hill, North Carolina 275995

Received 11 February 1999/Accepted 13 April 1999

The uspA1 and uspA2 genes of M. catarrhalis O35E encode two different surface-exposed proteins which were previously shown to share a 140-amino-acid region with 93% identity (C. Aebi, I. Maciver, J. L. Latimer, L. D. Cope, M. K. Stevens, S. E. Thomas, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 65:4367-4377, 1997). The N-terminal amino acid sequences of the mature forms of both UspA1 and UspA2 from strain O35E were determined after enzymatic treatment to remove the N-terminal pyroglutamyl residue that had blocked Edman degradation. Mass spectrometric analysis indicated that the molecular mass of UspA1 from M. catarrhalis O35E was 83,500 ± 116 Da. Nucleotide sequence analysis of the uspA1 and uspA2 genes from three other M. catarrhalis strains (TTA24, ATCC 25238, and V1171) revealed that the encoded protein products were very similar to those from strain O35E. Western blot analysis was used to confirm that each of these three strains of M. catarrhalis expressed both UspA1 and UspA2 proteins. Several different and repetitive amino acid motifs were present in both UspA1 and UspA2 from these four strains, and some of these were predicted to form coiled coils. Linear DNA templates were used in an in vitro transcription-translation system to determine the sizes of the monomeric forms of the UspA1 and UspA2 proteins from strains O35E and TTA24.

* Corresponding author. Mailing address: Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9048. Phone: (214) 648-5974. Fax: (214) 648-5905. E-mail: hansen01{at}utsw.swmed.edu.

Journal of Bacteriology, July 1999, p. 4026-4034, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Brooks, M. J., Sedillo, J. L., Wagner, N., Laurence, C. A., Wang, W., Attia, A. S., Hansen, E. J., Gray-Owen, S. D. (2008). Modular Arrangement of Allelic Variants Explains the Divergence in Moraxella catarrhalis UspA Protein Function. Infect. Immun. 76: 5330-5340 [Abstract] [Full Text]  
  • Brooks, M. J., Sedillo, J. L., Wagner, N., Wang, W., Attia, A. S., Wong, H., Laurence, C. A., Hansen, E. J., Gray-Owen, S. D. (2008). Moraxella catarrhalis Binding to Host Cellular Receptors Is Mediated by Sequence-Specific Determinants Not Conserved among All UspA1 Protein Variants. Infect. Immun. 76: 5322-5329 [Abstract] [Full Text]  
  • Ackermann, N., Tiller, M., Anding, G., Roggenkamp, A., Heesemann, J. (2008). Contribution of Trimeric Autotransporter C-Terminal Domains of Oligomeric Coiled-Coil Adhesin (Oca) Family Members YadA, UspA1, EibA, and Hia to Translocation of the YadA Passenger Domain and Virulence of Yersinia enterocolitica. J. Bacteriol. 190: 5031-5043 [Abstract] [Full Text]  
  • Valle, J., Mabbett, A. N., Ulett, G. C., Toledo-Arana, A., Wecker, K., Totsika, M., Schembri, M. A., Ghigo, J.-M., Beloin, C. (2008). UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli. J. Bacteriol. 190: 4147-4161 [Abstract] [Full Text]  
  • Manolov, T., Tan, T. T., Forsgren, A., Riesbeck, K. (2008). Moraxella-Dependent {alpha}1-Antichymotrypsin Neutralization: A Unique Virulence Mechanism. Am. J. Respir. Cell Mol. Bio. 38: 609-617 [Abstract] [Full Text]  
  • Grosskinsky, U., Schutz, M., Fritz, M., Schmid, Y., Lamparter, M. C., Szczesny, P., Lupas, A. N., Autenrieth, I. B., Linke, D. (2007). A Conserved Glycine Residue of Trimeric Autotransporter Domains Plays a Key Role in Yersinia Adhesin A Autotransport. J. Bacteriol. 189: 9011-9019 [Abstract] [Full Text]  
  • Luke, N. R., Jurcisek, J. A., Bakaletz, L. O., Campagnari, A. A. (2007). Contribution of Moraxella catarrhalis Type IV Pili to Nasopharyngeal Colonization and Biofilm Formation. Infect. Immun. 75: 5559-5564 [Abstract] [Full Text]  
  • Balder, R., Hassel, J., Lipski, S., Lafontaine, E. R. (2007). Moraxella catarrhalis Strain O35E Expresses Two Filamentous Hemagglutinin-Like Proteins That Mediate Adherence to Human Epithelial Cells. Infect. Immun. 75: 2765-2775 [Abstract] [Full Text]  
  • Wang, W., Pearson, M. M., Attia, A. S., Blick, R. J., Hansen, E. J. (2007). A UspA2H-Negative Variant of Moraxella catarrhalis Strain O46E Has a Deletion in a Homopolymeric Nucleotide Repeat Common to uspA2H Genes. Infect. Immun. 75: 2035-2045 [Abstract] [Full Text]  
  • Brooks, M. J., Laurence, C. A., Hansen, E. J., Gray-Owen, S. D. (2007). Characterization of the Moraxella catarrhalis Opa-Like Protein, OlpA, Reveals a Phylogenetically Conserved Family of Outer Membrane Proteins. J. Bacteriol. 189: 76-82 [Abstract] [Full Text]  
  • Lipski, S. L., Akimana, C., Timpe, J. M., Wooten, R. M., Lafontaine, E. R. (2007). The Moraxella catarrhalis Autotransporter McaP Is a Conserved Surface Protein That Mediates Adherence to Human Epithelial Cells through Its N-Terminal Passenger Domain. Infect. Immun. 75: 314-324 [Abstract] [Full Text]  
  • Attia, A. S., Hansen, E. J. (2006). A Conserved Tetranucleotide Repeat Is Necessary for Wild-Type Expression of the Moraxella catarrhalis UspA2 Protein. J. Bacteriol. 188: 7840-7852 [Abstract] [Full Text]  
  • Tan, T. T., Christensen, J. J., Dziegiel, M. H., Forsgren, A., Riesbeck, K. (2006). Comparison of the Serological Responses to Moraxella catarrhalis Immunoglobulin D-Binding Outer Membrane Protein and the Ubiquitous Surface Proteins A1 and A2. Infect. Immun. 74: 6377-6386 [Abstract] [Full Text]  
  • Cotter, S. E., Surana, N. K., Grass, S., St. Geme, J. W. III (2006). Trimeric autotransporters require trimerization of the passenger domain for stability and adhesive activity.. J. Bacteriol. 188: 5400-5407 [Abstract] [Full Text]  
  • Pearson, M. M., Laurence, C. A., Guinn, S. E., Hansen, E. J. (2006). Biofilm Formation by Moraxella catarrhalis In Vitro: Roles of the UspA1 Adhesin and the Hag Hemagglutinin. Infect. Immun. 74: 1588-1596 [Abstract] [Full Text]  
  • Bullard, B., Lipski, S. L., Lafontaine, E. R. (2005). Hag Directly Mediates the Adherence of Moraxella catarrhalis to Human Middle Ear Cells. Infect. Immun. 73: 5127-5136 [Abstract] [Full Text]  
  • Attia, A. S., Lafontaine, E. R., Latimer, J. L., Aebi, C., Syrogiannopoulos, G. A., Hansen, E. J. (2005). The UspA2 Protein of Moraxella catarrhalis Is Directly Involved in the Expression of Serum Resistance. Infect. Immun. 73: 2400-2410 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
  • Nordstrom, T., Blom, A. M., Forsgren, A., Riesbeck, K. (2004). The Emerging Pathogen Moraxella catarrhalis Interacts with Complement Inhibitor C4b Binding Protein through Ubiquitous Surface Proteins A1 and A2. J. Immunol. 173: 4598-4606 [Abstract] [Full Text]  
  • Zhang, P., Chomel, B. B., Schau, M. K., Goo, J. S., Droz, S., Kelminson, K. L., George, S. S., Lerche, N. W., Koehler, J. E. (2004). A family of variably expressed outer-membrane proteins (Vomp) mediates adhesion and autoaggregation in Bartonella quintana. Proc. Natl. Acad. Sci. USA 101: 13630-13635 [Abstract] [Full Text]  
  • Holm, M. M., Vanlerberg, S. L., Foley, I. M., Sledjeski, D. D., Lafontaine, E. R. (2004). The Moraxella catarrhalis Porin-Like Outer Membrane Protein CD Is an Adhesin for Human Lung Cells. Infect. Immun. 72: 1906-1913 [Abstract] [Full Text]  
  • Holm, M. M., Vanlerberg, S. L., Sledjeski, D. D., Lafontaine, E. R. (2003). The Hag Protein of Moraxella catarrhalis Strain O35E Is Associated with Adherence to Human Lung and Middle Ear Cells. Infect. Immun. 71: 4977-4984 [Abstract] [Full Text]  
  • Timpe, J. M., Holm, M. M., Vanlerberg, S. L., Basrur, V., Lafontaine, E. R. (2003). Identification of a Moraxella catarrhalis Outer Membrane Protein Exhibiting Both Adhesin and Lipolytic Activities. Infect. Immun. 71: 4341-4350 [Abstract] [Full Text]  
  • Roggenkamp, A., Ackermann, N., Jacobi, C. A., Truelzsch, K., Hoffmann, H., Heesemann, J. (2003). Molecular Analysis of Transport and Oligomerization of the Yersinia enterocolitica Adhesin YadA. J. Bacteriol. 185: 3735-3744 [Abstract] [Full Text]  
  • Forsgren, A., Brant, M., Karamehmedovic, M., Riesbeck, K. (2003). The Immunoglobulin D-Binding Protein MID from Moraxella catarrhalis Is Also an Adhesin. Infect. Immun. 71: 3302-3309 [Abstract] [Full Text]  
  • Ward, C. K., Latimer, J. L., Nika, J., Vakevainen, M., Mock, J. R., Deng, K., Blick, R. J., Hansen, E. J. (2003). Mutations in the lspA1 and lspA2 Genes of Haemophilus ducreyi Affect the Virulence of This Pathogen in an Animal Model System. Infect. Immun. 71: 2478-2486 [Abstract] [Full Text]  
  • Simpson, A. E., Skurray, R. A., Firth, N. (2003). A Single Gene on the Staphylococcal Multiresistance Plasmid pSK1 Encodes a Novel Partitioning System. J. Bacteriol. 185: 2143-2152 [Abstract] [Full Text]  
  • Mollenkvist, A., Nordstrom, T., Hallden, C., Christensen, J. J., Forsgren, A., Riesbeck, K. (2003). The Moraxella catarrhalis Immunoglobulin D-Binding Protein MID Has Conserved Sequences and Is Regulated by a Mechanism Corresponding to Phase Variation. J. Bacteriol. 185: 2285-2295 [Abstract] [Full Text]  
  • Pearson, M. M., Lafontaine, E. R., Wagner, N. J., St. Geme III, J. W., Hansen, E. J. (2002). A hag Mutant of Moraxella catarrhalis Strain O35E Is Deficient in Hemagglutination, Autoagglutination, and Immunoglobulin D-Binding Activities. Infect. Immun. 70: 4523-4533 [Abstract] [Full Text]  
  • Comanducci, M., Bambini, S., Brunelli, B., Adu-Bobie, J., Arico, B., Capecchi, B., Giuliani, M. M., Masignani, V., Santini, L., Savino, S., Granoff, D. M., Caugant, D. A., Pizza, M., Rappuoli, R., Mora, M. (2002). NadA, a Novel Vaccine Candidate of Neisseria meningitidis. JEM 195: 1445-1454 [Abstract] [Full Text]  
  • Smoot, L. M., Franke, D. D., McGillivary, G., Actis, L. A. (2002). Genomic Analysis of the F3031 Brazilian Purpuric Fever Clone of Haemophilus influenzae Biogroup Aegyptius by PCR-Based Subtractive Hybridization. Infect. Immun. 70: 2694-2699 [Abstract] [Full Text]  
  • Verduin, C. M., Hol, C., Fleer, A., van Dijk, H., van Belkum, A. (2002). Moraxella catarrhalis: from Emerging to Established Pathogen. Clin. Microbiol. Rev. 15: 125-144 [Abstract] [Full Text]  
  • Forsgren, A., Brant, M., Mollenkvist, A., Muyombwe, A., Janson, H., Woin, N., Riesbeck, K. (2001). Isolation and Characterization of a Novel IgD-Binding Protein from Moraxella catarrhalis. J. Immunol. 167: 2112-2120 [Abstract] [Full Text]  
  • Sethi, S., Murphy, T. F. (2001). Bacterial Infection in Chronic Obstructive Pulmonary Disease in 2000: a State-of-the-Art Review. Clin. Microbiol. Rev. 14: 336-363 [Abstract] [Full Text]  
  • Henderson, I. R., Nataro, J. P. (2001). Virulence Functions of Autotransporter Proteins. Infect. Immun. 69: 1231-1243 [Full Text]  
  • Lafontaine, E. R., Wagner, N. J., Hansen, E. J. (2001). Expression of the Moraxella catarrhalis UspA1 Protein Undergoes Phase Variation and Is Regulated at the Transcriptional Level. J. Bacteriol. 183: 1540-1551 [Abstract] [Full Text]  
  • St. Geme, J. W. III, Cutter, D. (2000). The Haemophilus influenzae Hia Adhesin Is an Autotransporter Protein That Remains Uncleaved at the C Terminus and Fully Cell Associated. J. Bacteriol. 182: 6005-6013 [Abstract] [Full Text]  
  • Lafontaine, E. R., Cope, L. D., Aebi, C., Latimer, J. L., McCracken, G. H. Jr., Hansen, E. J. (2000). The UspA1 Protein and a Second Type of UspA2 Protein Mediate Adherence of Moraxella catarrhalis to Human Epithelial Cells In Vitro. J. Bacteriol. 182: 1364-1373 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»