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Journal of Bacteriology, July 1999, p. 4035-4040, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Extracellular Xylan Degradative System in Clostridium cellulolyticum Cultivated on Xylan: Evidence for Cell-Free Cellulosome Production

O. Mohand-Oussaid,1 S. Payot,2 E. Guedon,2 E. Gelhaye,2,* A. Youyou,1 and H. Petitdemange2

Laboratoire de Microbiologie URBAF, Institut des Sciences de la Nature, Université Mouloud Mammeri, Tizi-Ouzou, Algeria,1 and Laboratoire de Biochimie des Bactéries Gram +, Université Henri Poincaré, Vandoeuvre-lès-Nancy, France2

Received 11 January 1999/Accepted 27 April 1999

In this study, we demonstrate that the cellulosome of Clostridium cellulolyticum grown on xylan is not associated with the bacterial cell. Indeed, the large majority of the activity (about 90%) is localized in the cell-free fraction when the bacterium is grown on xylan. Furthermore, about 70% of the detected xylanase activity is associated with cell-free high-molecular-weight complexes containing avicelase activity and the cellulosomal scaffolding protein CipC. The same repartition is observed with carboxymethyl cellulase activity. The cellulose adhesion of xylan-grown cells is sharply reduced in comparison with cellulose-grown cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that cellulosomes derived from xylan- and cellulose-grown cells have different compositions. In both cases, the scaffolding protein CipC is present, but the relative proportions of the other components is dramatically changed depending on the growth substrate. We propose that, depending on the growth substrate, C. cellulolyticum is able to regulate the cell association and cellulose adhesion of cellulosomes and regulate cellulosomal composition.

* Corresponding author. Mailing address: Laboratoire de Biochimie des Bactéries Gram +, Université Henri Poincaré, Faculté des Sciences, B.P. 239, 54506 Vandoeuvre-lès-Nancy Cedex, France. Phone: 33 3 83 91 20 53. Fax: 33 3 83 91 25 50. E-mail: gelhaye{at}lcb.u-nancy.fr.

Journal of Bacteriology, July 1999, p. 4035-4040, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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