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Journal of Bacteriology, July 1999, p. 4103-4105, Vol. 181, No. 13
Faculty of Pharmaceutical Sciences, Chiba
University, Inage-ku, Chiba 263-8522, Japan
Received 6 January 1999/Accepted 28 April 1999
A K+/H+ antiporter regulates cytoplasmic pH
in Enterococcus hirae growing at alkaline pH. Mutants
defective in this antiport activity were alkaline pH sensitive. One
mutant, Pop1, lacked both K+/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs
below 8 but did not at pHs above 9, conditions under which cytoplasmic
pH was not fully acidified.
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Isolation and Properties of Enterococcus
hirae Mutants Defective in the Potassium/Proton Antiport
System
*
Corresponding author. Mailing address: Faculty of
Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku,
Chiba 263-8522, Japan. Phone: 81-43-290-2898. Fax: 81-43-290-2900. E-mail: yoshimi{at}athenaeum.p.chiba-u.ac.jp.
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