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Journal of Bacteriology, July 1999, p. 4118-4124, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The pvc Gene Cluster of Pseudomonas aeruginosa: Role in Synthesis of the Pyoverdine Chromophore and Regulation by PtxR and PvdS

Alain Stintzi,1,2 Zaiga Johnson,3 Martin Stonehouse,3 Urs Ochsner,3 Jean-Marie Meyer,2 Michael L. Vasil,3 and Keith Poole1,*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N61; Laboratoire de Microbiologie et de Génétique, Unité de Recherche Associée au Centre National de la Recherche Scientifique No. 1481, Université Louis-Pasteur, 67000 Strasbourg, France2; and Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 802623

Received 8 March 1999/Accepted 28 April 1999

A putative operon of four genes implicated in the synthesis of the chromophore moiety of the Pseudomonas aeruginosa siderophore pyoverdine, dubbed pvcABCD (where pvc stands for pyoverdine chromophore), was cloned and sequenced. Mutational inactivation of the pvc genes abrogated pyoverdine biosynthesis, consistent with their involvement in the biosynthesis of this siderophore. pvcABCD expression was negatively regulated by iron and positively regulated by both PvdS, the alternate sigma factor required for pyoverdine biosynthesis, and PtxR, a LysR family activator previously implicated in exotoxin A regulation.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6. Phone: (613) 545-6677. Fax: (613) 545-6796. E-mail: poolek{at}post.queensu.ca.

Journal of Bacteriology, July 1999, p. 4118-4124, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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