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Journal of Bacteriology, July 1999, p. 4129-4132, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Nitric Oxide Is a Signal for NNR-Mediated Transcription Activation in Paracoccus denitrificans

Rob J. M. Van Spanning,1,* Edith Houben,1 Willem N. M. Reijnders,1 Stephen Spiro,2 Hans V. Westerhoff,1 and Neil Saunders1

Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands,1 and School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England2

Received 8 February 1999/Accepted 26 April 1999

By using the 'lacZ gene, the activities of the nirI, nirS, and norC promoters were assayed in the wild type and in NNR-deficient mutants of Paracoccus denitrificans grown under various growth conditions. In addition, induction profiles of the three promoters in response to the presence of various nitrogenous oxides were determined. Transcription from the three promoters required the absence of oxygen and the presence both of the transcriptional activator NNR and of nitric oxide. The activity of the nnr promoter itself was halved after the cells had been switched from aerobic respiration to denitrification. This response was apparently not a result of autoregulation or of regulation by FnrP, since the nnr promoter was as active in the wild-type strain as it was in NNR- or FnrP-deficient mutants.

* Corresponding author. Mailing address: Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands. Phone: 31 20 4447179. Fax: 31 20 4447229. E-mail: spanning{at}bio.vu.nl.

Journal of Bacteriology, July 1999, p. 4129-4132, Vol. 181, No. 13
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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