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Journal of Bacteriology, July 1999, p. 4185-4192, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Site-Specific Recombination Genes int and xis of the Rhizobium Temperate Phage 16-3

Szabolcs Semsey,^1,2 IstvAn Papp,³ Zsuzsanna Buzas,⁴ Andras Patthy,⁴ Laszlo Orosz,^1,2 and Peter P. Papp^1,*

Institute for Molecular Genetics¹ and Institute for Biochemistry and Protein Research,⁴ Agricultural Biotechnology Center, Gödöll, H-2100 Hungary; Department of Biotechnology and Molecular Genetics, Gödöll University of Agricultural Sciences, Gödöll, H-2105 Hungary²; and Max-Planck Institut für Zuchtungsforschung, D-50829 Cologne, Germany³

Received 29 December 1998/Accepted 4 May 1999

Phage 16-3 is a temperate phage of Rhizobium meliloti 41 which integrates its genome with high efficiency into the host chromosome by site-specific recombination through DNA sequences of attB and attP. Here we report the identification of two phage-encoded genes required for recombinations at these sites: int (phage integration) and xis (prophage excision). We concluded that Int protein of phage 16-3 belongs to the integrase family of tyrosine recombinases. Despite similarities to the cognate systems of the lambdoid phages, the 16-3 int xis att system is not active in Escherichia coli, probably due to requirements for host factors that differ in Rhizobium meliloti and E. coli. The application of the 16-3 site-specific recombination system in biotechnology is discussed.

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^* Corresponding author. Mailing address: Institute for Molecular Genetics, Agricultural Biotechnology Center, Szent-Györgyi A. 4, Gödöll, H-2100 Hungary. Phone: 36 (28) 430-600. Fax: 36 (28) 430-416. E-mail: ppapp{at}abc.hu.

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Journal of Bacteriology, July 1999, p. 4185-4192, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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