Structural and Functional Analyses of Photosynthetic Regulatory Genes regA and regB from Rhodovulum sulfidophilum, Roseobacter denitrificans, and Rhodobacter capsulatus

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Journal of Bacteriology, July 1999, p. 4205-4215, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Structural and Functional Analyses of Photosynthetic Regulatory Genes regA and regB from Rhodovulum sulfidophilum, Roseobacter denitrificans, and Rhodobacter capsulatus

Shinji Masuda,¹ Yumi Matsumoto,¹ Kenji V. P. Nagashima,¹ Keizo Shimada,¹ Kazuhito Inoue,² Carl E. Bauer,³ and Katsumi Matsuura¹^,^*

Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397,¹ and Department of Biological Sciences, Kanagawa University, Tsuchiya, Hiratsuka, Kanagawa, 259-1293,² Japan, and Department of Biology, Indiana University, Bloomington, Indiana 47405³

Received 11 March 1999/Accepted 5 May 1999

Genes coding for putative RegA, RegB, and SenC homologues were identified and characterized in the purple nonsulfur photosyntheticbacteria Rhodovulum sulfidophilum and Roseobacter denitrificans,species that demonstrate weak or no oxygen repression of photosystemsynthesis. This additional sequence information was then usedto perform a comparative analysis with previously sequenced RegA,RegB, and SenC homologues obtained from Rhodobacter capsulatusand Rhodobacter sphaeroides. These are photosynthetic bacteriathat exhibit a high level of oxygen repression of photosystemsynthesis controlled by the RegA-RegB two-component regulatorysystem. The response regulator, RegA, exhibits a remarkable 78.7to 84.2% overall sequence identity, with total conservation withina putative helix-turn-helix DNA-binding motif. The RegB sensorkinase homologues also exhibit a high level of sequence conservation(55.9 to 61.5%) although these additional species give significantlydifferent responses to oxygen. A Rhodovulum sulfidophilum mutantlacking regA or regB was constructed. These mutants produced smalleramounts of photopigments under aerobic and anaerobic conditions,indicating that the RegA-RegB regulon controls photosyntheticgene expression in this bacterium as it does as in Rhodobacterspecies. Rhodobacter capsulatus regA- or regB-deficient mutantsrecovered the synthesis of a photosynthetic apparatus that stillretained regulation by oxygen tension when complemented with reggenes from Rhodovulum sulfidophilum and Roseobacter denitrificans.These results suggest that differential expression of photosyntheticgenes in response to aerobic and anaerobic growth conditions isnot the result of altered redox sensing by the sensor kinase protein,RegB.

^* Corresponding author. Mailing address: Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo192-0397, Japan. Phone: 81-426-77-2582. Fax: 81-426-77-2559. E-mail:matsuura-katsumi{at}c.metro-u.ac.jp.

Journal of Bacteriology, July 1999, p. 4205-4215, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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