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Journal of Bacteriology, July 1999, p. 4205-4215, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Structural and Functional Analyses of
Photosynthetic Regulatory Genes regA and regB
from Rhodovulum sulfidophilum, Roseobacter
denitrificans, and Rhodobacter capsulatus
Shinji
Masuda,1
Yumi
Matsumoto,1
Kenji V. P.
Nagashima,1
Keizo
Shimada,1
Kazuhito
Inoue,2
Carl E.
Bauer,3 and
Katsumi
Matsuura1,*
Department of Biology, Tokyo Metropolitan
University, Minamiohsawa, Hachioji, Tokyo
192-0397,1 and Department of Biological
Sciences, Kanagawa University, Tsuchiya, Hiratsuka, Kanagawa,
259-1293,2 Japan, and Department of
Biology, Indiana University, Bloomington, Indiana
474053
Received 11 March 1999/Accepted 5 May 1999
Genes coding for putative RegA, RegB, and SenC homologues were
identified and characterized in the purple nonsulfur photosynthetic bacteria Rhodovulum sulfidophilum and Roseobacter
denitrificans, species that demonstrate weak or no oxygen
repression of photosystem synthesis. This additional sequence
information was then used to perform a comparative analysis with
previously sequenced RegA, RegB, and SenC homologues obtained from
Rhodobacter capsulatus and Rhodobacter
sphaeroides. These are photosynthetic bacteria that exhibit a
high level of oxygen repression of photosystem synthesis controlled by
the RegA-RegB two-component regulatory system. The response regulator,
RegA, exhibits a remarkable 78.7 to 84.2% overall sequence identity,
with total conservation within a putative helix-turn-helix DNA-binding
motif. The RegB sensor kinase homologues also exhibit a high level of
sequence conservation (55.9 to 61.5%) although these additional
species give significantly different responses to oxygen. A
Rhodovulum sulfidophilum mutant lacking regA or
regB was constructed. These mutants produced smaller amounts of photopigments under aerobic and anaerobic conditions, indicating that the RegA-RegB regulon controls photosynthetic gene
expression in this bacterium as it does as in Rhodobacter species. Rhodobacter capsulatus regA- or
regB-deficient mutants recovered the synthesis of a
photosynthetic apparatus that still retained regulation by oxygen
tension when complemented with reg genes from
Rhodovulum sulfidophilum and Roseobacter
denitrificans. These results suggest that differential expression
of photosynthetic genes in response to aerobic and anaerobic growth
conditions is not the result of altered redox sensing by the sensor
kinase protein, RegB.
*
Corresponding author. Mailing address: Department of
Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan. Phone: 81-426-77-2582. Fax: 81-426-77-2559. E-mail: matsuura-katsumi{at}c.metro-u.ac.jp.
Journal of Bacteriology, July 1999, p. 4205-4215, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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