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Journal of Bacteriology, July 1999, p. 4411-4416, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Organization of the citCDEF Locus and Identification of mae and clyR Genes from Leuconostoc mesenteroides

Sadjia Bekal-Si Ali, Charles Diviès, and Hervé Prévost*

Laboratoire de Microbiologie, UA INRA, Université de Bourgogne ENS.BANA, F-21 000 Dijon, France

Received 8 February 1999/Accepted 12 May 1999

In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the citrate lyase locus revealed one polycistronic mRNA covering the mae and citCDEF genes. This transcript was detected only on RNA prepared from cells grown in the presence of citrate. Primer extension experiments suggest that clyR and the citrate lyase operon are expressed from a bidirectional A-T-rich promoter region located between mae and clyR.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, UA INRA, Université de Bourgogne ENS.BANA, 1 Esplanade Erasme, F-21 000 Dijon, France. Phone and Fax: 33 3 80 39 66 40. E-mail: hprevost{at}u-bourgogne.fr.

Journal of Bacteriology, July 1999, p. 4411-4416, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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