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Journal of Bacteriology, July 1999, p. 4424-4429, Vol. 181, No. 14
Department of Microbiology, The Ohio State
University, Columbus, Ohio 432101;
Institut für Allgemeine Mikrobiologie, Universität
Kiel, 24188 Kiel, Germany2; and Banting
and Best Department of Medical Research, University of Toronto,
Toronto, Ontario M5G 1L6, Canada3
Received 16 February 1999/Accepted 27 April 1999
RNA polymerase (RNAP) purified from Methanobacterium
thermoautotrophicum
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Methanobacterium thermoautotrophicum RNA
Polymerase and Transcription In Vitro
H has been shown to initiate transcription
accurately in vitro from the hmtB archaeal histone promoter
with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor
TFB. Efforts to obtain transcription initiation from hydrogen-regulated
methane gene promoters were, however, unsuccessful. Two previously
unrecognized archaeal RNAP subunits have been identified, and complex
formation by the M. thermoautotrophicum RNAP and TFB has
been demonstrated.
*
Corresponding author. Mailing address: Department of
Microbiology, The Ohio State University, Columbus, OH 43210. Phone:
(614) 292-2301. Fax: (614) 292-8120. E-mail:
reeve.2{at}osu.edu.
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