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Journal of Bacteriology, July 1999, p. 4424-4429, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Methanobacterium thermoautotrophicum RNA Polymerase and Transcription In Vitro

Trevor J. Darcy,1 Winfried Hausner,2 Donald E. Awery,3 Aled M. Edwards,3 Michael Thomm,2 and John N. Reeve1,*

Department of Microbiology, The Ohio State University, Columbus, Ohio 432101; Institut für Allgemeine Mikrobiologie, Universität Kiel, 24188 Kiel, Germany2; and Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada3

Received 16 February 1999/Accepted 27 April 1999

RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum Delta H has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor TFB. Efforts to obtain transcription initiation from hydrogen-regulated methane gene promoters were, however, unsuccessful. Two previously unrecognized archaeal RNAP subunits have been identified, and complex formation by the M. thermoautotrophicum RNAP and TFB has been demonstrated.

* Corresponding author. Mailing address: Department of Microbiology, The Ohio State University, Columbus, OH 43210. Phone: (614) 292-2301. Fax: (614) 292-8120. E-mail: reeve.2{at}osu.edu.

Journal of Bacteriology, July 1999, p. 4424-4429, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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