Journal of Bacteriology, July 1999, p. 4435-4436, Vol. 181, No. 14
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Biology Department, Concordia University, Montréal, Québec, Canada H36 1M8
Received 19 January 1999/Accepted 26 April 1999
Using rabbit polyclonal antibodies, we have shown that the Dcm cytosine methylase of Escherichia coli is maintained at a constant level during cell growth, while Vsr endonuclease levels are growth phase dependent. Decreased production of Vsr relative to Dcm during the log phase may contribute substantially to the mutability of 5-methylcytosine.
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