This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meijer, A.
Right arrow Articles by Ossewaarde, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meijer, A.
Right arrow Articles by Ossewaarde, J. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1999, p. 4469-4475, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genomic Relatedness of Chlamydia Isolates Determined by Amplified Fragment Length Polymorphism Analysis

Adam Meijer,1,* Servaas A. Morré,2 Adriaan J. C. Van Den Brule,2 Paul H. M. Savelkoul,3 and Jacobus M. Ossewaarde1

Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, 3720 BA Bilthoven,1 and Section of Molecular Pathology, Department of Pathology,2 and Department of Clinical Microbiology and Infection Control,3 University Hospital Vrije Universiteit, 1081 HV Amsterdam, The Netherlands

Received 21 December 1998/Accepted 22 April 1999

The genomic relatedness of 19 Chlamydia pneumoniae isolates (17 from respiratory origin and 2 from atherosclerotic origin), 21 Chlamydia trachomatis isolates (all serovars from the human biovar, an isolate from the mouse biovar, and a porcine isolate), 6 Chlamydia psittaci isolates (5 avian isolates and 1 feline isolate), and 1 Chlamydia pecorum isolate was studied by analyzing genomic amplified fragment length polymorphism (AFLP) fingerprints. The AFLP procedure was adapted from a previously developed method for characterization of clinical C. trachomatis isolates. The fingerprints of all C. pneumoniae isolates were nearly identical, clustering together at a Dice similarity of 92.6% (± 1.6% standard deviation). The fingerprints of the C. trachomatis isolates of human, mouse, and swine origin were clearly distinct from each other. The fingerprints of the isolates from the human biovar could be divided into at least 12 different types when the presence or absence of specific bands was taken into account. The C. psittaci fingerprints could be divided into a parakeet, a pigeon, and a feline type. The fingerprint of C. pecorum was clearly distinct from all others. Cluster analysis of selected isolates from all species revealed groups other than those based on sequence data from single genes (in particular, omp1 and rRNA genes) but was in agreement with available DNA-DNA hybridization data. In conclusion, cluster analysis of AFLP fingerprints of representatives of all species provided suggestions for a grouping of chlamydiae based on the analysis of the whole genome. Furthermore, genomic AFLP analysis showed that the genome of C. pneumoniae is highly conserved and that no differences exist between isolates of respiratory and atherosclerotic origins.

* Corresponding author. Mailing address: Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31 30 2743595. Fax: 31 30 2744449. E-mail: Adam.Meijer{at}rivm.nl.

Journal of Bacteriology, August 1999, p. 4469-4475, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Quint, K. D., van Doorn, L.-J., Kleter, B., de Koning, M. N.C., van den Munckhof, H. A.M., Morre, S. A., ter Harmsel, B., Weiderpass, E., Harbers, G., Melchers, W. J.G., Quint, W. G.V. (2007). A Highly Sensitive, Multiplex Broad-Spectrum PCR-DNA-Enzyme Immunoassay and Reverse Hybridization Assay for Rapid Detection and Identification of Chlamydia trachomatis Serovars. J. Mol. Diagn. 9: 631-638 [Abstract] [Full Text]  
  • de Kruif, M. D., van Gorp, E. C.M., Keller, T. T., Ossewaarde, J. M., ten Cate, H. (2005). Chlamydia pneumoniae infections in mouse models: relevance for atherosclerosis research. Cardiovasc Res 65: 317-327 [Abstract] [Full Text]  
  • Fournier, P.-E., Zhu, Y., Ogata, H., Raoult, D. (2004). Use of Highly Variable Intergenic Spacer Sequences for Multispacer Typing of Rickettsia conorii Strains. J. Clin. Microbiol. 42: 5757-5766 [Abstract] [Full Text]  
  • Van Loock, M., Vanrompay, D., Herrmann, B., Vander Stappen, J., Volckaert, G., Goddeeris, B. M., Everett, K. D. E. (2003). Missing links in the divergence of Chlamydophila abortus from Chlamydophila psittaci. Int. J. Syst. Evol. Microbiol. 53: 761-770 [Abstract] [Full Text]  
  • Griffiths, E., Gupta, R. S. (2002). Protein signatures distinctive of chlamydial species: horizontal transfers of cell wall biosynthesis genes glmU from archaea to chlamydiae and murA between chlamydiae and Streptomyces. Microbiology 148: 2541-2549 [Abstract] [Full Text]  
  • Pannekoek, Y., van der Ende, A., Eijk, P. P., van Marle, J., de Witte, M. A., Ossewaarde, J. M., van den Brule, A. J. C., Morre, S. A., Dankert, J. (2001). Normal IncA Expression and Fusogenicity of Inclusions in Chlamydia trachomatis Isolates with the incA I47T Mutation. Infect. Immun. 69: 4654-4656 [Abstract] [Full Text]  
  • Morre, S. A., Lyons, J. M., Ito, J. I. Jr., Morrison, R. P. (2000). Murine Models of Chlamydia trachomatis Genital Tract Infection: Use of Mouse Pneumonitis Strain versus Human Strains. Infect. Immun. 68: 7209-7211 [Full Text]  
  • Morré, S. A., Ossewaarde, J. M., Savelkoul, P. H. M., Stoof, J., Meijer, C. J. L. M., van den Brule, A. J. C. (2000). Analysis of Genetic Heterogeneity in Chlamydia trachomatis Clinical Isolates of Serovars D, E, and F by Amplified Fragment Length Polymorphism. J. Clin. Microbiol. 38: 3463-3466 [Abstract] [Full Text]  
  • Shirai, M., Hirakawa, H., Kimoto, M., Tabuchi, M., Kishi, F., Ouchi, K., Shiba, T., Ishii, K., Hattori, M., Kuhara, S., Nakazawa, T. (2000). Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA. Nucleic Acids Res 28: 2311-2314 [Abstract] [Full Text]  
  • Read, T. D., Brunham, R. C., Shen, C., Gill, S. R., Heidelberg, J. F., White, O., Hickey, E. K., Peterson, J., Utterback, T., Berry, K., Bass, S., Linher, K., Weidman, J., Khouri, H., Craven, B., Bowman, C., Dodson, R., Gwinn, M., Nelson, W., DeBoy, R., Kolonay, J., McClarty, G., Salzberg, S. L., Eisen, J., Fraser, C. M. (2000). Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39. Nucleic Acids Res 28: 1397-1406 [Abstract] [Full Text]  
  • Savelkoul, P. H. M., Aarts, H. J. M., de Haas, J., Dijkshoorn, L., Duim, B., Otsen, M., Rademaker, J. L. W., Schouls, L., Lenstra, J. A. (1999). Amplified-Fragment Length Polymorphism Analysis: the State of an Art. J. Clin. Microbiol. 37: 3083-3091 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»