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Journal of Bacteriology, August 1999, p. 4696-4699, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Cloning, Sequence Analysis, and Characterization of a Penicillin-Resistant DD-Carboxypeptidase of Myxococcus xanthus

Yoshio Kimura,* Yukie Takashima, Yushi Tokumasu, and Masayuki Sato

Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan

Received 11 March 1999/Accepted 20 May 1999

We have cloned a gene, pdcA, from the genomic library of Myxococcus xanthus with an oligonucleotide probe representing conserved regions of penicillin-resistant DD-carboxypeptidases. The amino- and carboxy-terminal halves of the predicted pdcA gene product showed significant sequence similarity to N-acetylmuramoyl-L-alanine amidase and penicillin-resistant DD-carboxypeptidase, respectively. The pdcA gene was expressed in Escherichia coli, and the characteristics of the gene product were similar to those of DD-carboxypeptidase (VanY) of vancomycin-resistant enterococci. No apparent changes in cell growth, sporulation, or germination were observed in pdcA deletion mutants.

* Corresponding author. Mailing address: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-Cho, Kagawa 761-0795, Japan. Phone: 81-87-891-3118. Fax: 81-87-891-3021. E-mail: kimura{at}ag.kagawa-u.ac.jp.

Journal of Bacteriology, August 1999, p. 4696-4699, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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