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Journal of Bacteriology, August 1999, p. 4700-4703, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Three Distinct Phases of Isoprene Formation during Growth and Sporulation of Bacillus subtilis

William P. Wagner, Michele Nemecek-Marshall, and Ray Fall*

Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215

Received 8 March 1999/Accepted 24 May 1999

During growth on a glucose-tryptone medium, Bacillus subtilis 6051 (Marburg strain) exhibited three phases of isoprene (2-methyl-1,3-butadiene) formation, corresponding to (i) glucose catabolism and secretion of acetoin, (ii) catabolism of acetoin, and (iii) the early stages of sporulation. These results establish an experimental system for studying the biological role of isoprene formation.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215. Phone: (303) 492-7914. Fax: (303) 492-1149. E-mail: fall{at}terra.colorado.edu.

Journal of Bacteriology, August 1999, p. 4700-4703, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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