This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adhikari, P.
Right arrow Articles by Verma, N. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adhikari, P.
Right arrow Articles by Verma, N. K.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1999, p. 4711-4718, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Serotype 1a O-Antigen Modification: Molecular Characterization of the Genes Involved and Their Novel Organization in the Shigella flexneri Chromosome

Pradip Adhikari,dagger Gwen Allison, Belinda Whittle, and Naresh K. Verma*

Division of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia

Received 15 March 1999/Accepted 7 May 1999

The factors responsible for serotype 1a O-antigen modification in Shigella flexneri were localized to a 5.8-kb chromosomal HindIII fragment of serotype 1a strain Y53. The entire 5.8-kb fragment and regions up- and downstream of it (10.6-kb total) were sequenced. A putative three-gene operon, which showed homology with other serotype conversion genes, was identified and shown to confer serotype 1a O-antigen modification. The serotype conversion genes were flanked on either side by phage DNA. Multiple insertion sequence (IS) elements were located within and upstream of the phage DNA in a composite transposon-like structure. Host DNA homologous to the dsdC and the thrW proA genes was located upstream of the IS elements and downstream of the phage DNA, respectively. The sequence analysis indicates that the organization of the 10.6-kb region of the Y53 chromosome is unique and suggests that the serotype conversion genes were originally brought into the host by a bacteriophage. Several features of this region are also characteristic of pathogenicity islands.

* Corresponding author. Mailing address: Division of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia. Phone: 61 2 6249 2666. Fax: 61 2 6249 0313. E-mail: Naresh.Verma{at}anu.edu.au.

dagger Present address: Immunobiology Section, Therapeutic Goods Administration Laboratories, Woden, ACT, Australia.

Journal of Bacteriology, August 1999, p. 4711-4718, Vol. 181, No. 15
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Stagg, R. M., Tang, S.-S., Carlin, N. I. A., Talukder, K. A., Cam, P. D., Verma, N. K. (2009). A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c. J. Bacteriol. 191: 6612-6617 [Abstract] [Full Text]  
  • Kaluzny, K., Abeyrathne, P. D., Lam, J. S. (2007). Coexistence of Two Distinct Versions of O-Antigen Polymerase, Wzy-Alpha and Wzy-Beta, in Pseudomonas aeruginosa Serogroup O2 and Their Contributions to Cell Surface Diversity. J. Bacteriol. 189: 4141-4152 [Abstract] [Full Text]  
  • Zhou, S., Kile, A., Bechner, M., Place, M., Kvikstad, E., Deng, W., Wei, J., Severin, J., Runnheim, R., Churas, C., Forrest, D., Dimalanta, E. T., Lamers, C., Burland, V., Blattner, F. R., Schwartz, D. C. (2004). Single-Molecule Approach to Bacterial Genomic Comparisons via Optical Mapping. J. Bacteriol. 186: 7773-7782 [Abstract] [Full Text]  
  • Markine-Goriaynoff, N., Gillet, L., Van Etten, J. L., Korres, H., Verma, N., Vanderplasschen, A. (2004). Glycosyltransferases encoded by viruses. J. Gen. Virol. 85: 2741-2754 [Abstract] [Full Text]  
  • Korres, H., Verma, N. K. (2004). Topological Analysis of Glucosyltransferase GtrV of Shigella flexneri by a Dual Reporter System and Identification of a Unique Reentrant Loop. J. Biol. Chem. 279: 22469-22476 [Abstract] [Full Text]  
  • Parreira, V. R., Gyles, C. L. (2003). A Novel Pathogenicity Island Integrated Adjacent to the thrW tRNA Gene of Avian Pathogenic Escherichia coli Encodes a Vacuolating Autotransporter Toxin. Infect. Immun. 71: 5087-5096 [Abstract] [Full Text]  
  • Allison, G. E., Angeles, D., Tran-Dinh, N., Verma, N. K. (2002). Complete Genomic Sequence of SfV, a Serotype-Converting Temperate Bacteriophage of Shigellaflexneri. J. Bacteriol. 184: 1974-1987 [Abstract] [Full Text]  
  • Brooks, B. W., Robertson, R. H., Lutze-Wallace, C. L., Pfahler, W. (2001). Identification, Characterization, and Variation in Expression of Two Serologically Distinct O-Antigen Epitopes in Lipopolysaccharides of Campylobacter fetus Serotype A Strains. Infect. Immun. 69: 7596-7602 [Abstract] [Full Text]  
  • Adams, M. M., Allison, G. E., Verma, N. K. (2001). Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296. Microbiology 147: 851-860 [Abstract] [Full Text]  
  • Byl, C. V., Kropinski, A. M. (2000). Sequence of the Genome of Salmonella Bacteriophage P22. J. Bacteriol. 182: 6472-6481 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»