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Journal of Bacteriology, August 1999, p. 4734-4740, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An Unspliced Group I Intron in 23S rRNA Links Chlamydiales, Chloroplasts, and Mitochondria

Karin D. E. Everett,1,* Simona Kahane,2 Robin M. Bush,3 and Maureen G. Friedman2

Avian and Swine Respiratory Diseases Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 500101; Department of Virology, Faculty of Health Sciences, Ben Gurion University, Beer Sheva, Israel2; and Department of Ecology and Evolutionary Biology, University of California at Irvine, Irvine, California 926963

Received 8 February 1999/Accepted 20 May 1999

Chlamydia was the only genus in the order Chlamydiales until the recent characterization of Simkania negevensis ZT and Parachlamydia acanthamoebae strains. The present study of Chlamydiales 23S ribosomal DNA (rDNA) focuses on a naturally occurring group I intron in the I-CpaI target site of 23S rDNA from S. negevensis. The intron, SnLSU · 1, belonged to the IB4 structural subgroup and was most closely related to large ribosomal subunit introns that express single-motif, LAGLIDADG endonucleases in chloroplasts of algae and in mitochondria of amoebae. RT-PCR and electrophoresis of in vivo rRNA indicated that the intron was not spliced out of the 23S rRNA. The unspliced 658-nt intron is the first group I intron to be found in bacterial rDNA or rRNA, and it may delay the S. negevensis developmental replication cycle by affecting ribosomal function.

* Corresponding author. Present address: Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7371. Phone: (706) 583-0237 or 542-5823. Fax: (706) 542-5771. E-mail: keverett{at}calc.vet.uga.edu or kdeeverett{at}hotmail.com.

Journal of Bacteriology, August 1999, p. 4734-4740, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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