This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chaillou, S.
Right arrow Articles by Postma, P. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chaillou, S.
Right arrow Articles by Postma, P. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1999, p. 4768-4773, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transport of D-Xylose in Lactobacillus pentosus, Lactobacillus casei, and Lactobacillus plantarum: Evidence for a Mechanism of Facilitated Diffusion via the Phosphoenolpyruvate:Mannose Phosphotransferase System

Stéphane Chaillou,1,2 Peter H. Pouwels,1,2,* and Pieter W. Postma1

EC Slater Institute, BioCentrum, University of Amsterdam, 1018 TV Amsterdam,1 and Department of Molecular Genetics and Gene Technology, TNO Nutrition and Food Research Institute, 3700AJ Zeist,2 The Netherlands

Received 4 November 1998/Accepted 10 June 1999

We have identified and characterized the D-xylose transport system of Lactobacillus pentosus. Uptake of D-xylose was not driven by the proton motive force generated by malolactic fermentation and required D-xylose metabolism. The kinetics of D-xylose transport were indicative of a low-affinity facilitated-diffusion system with an apparent Km of 8.5 mM and a Vmax of 23 nmol min-1 mg of dry weight-1. In two mutants of L. pentosus defective in the phosphoenolpyruvate:mannose phosphotransferase system, growth on D-xylose was absent due to the lack of D-xylose transport. However, transport of the pentose was not totally abolished in a third mutant, which could be complemented after expression of the L. curvatus manB gene encoding the cytoplasmic EIIBMan component of the EIIMan complex. The EIIMan complex is also involved in D-xylose transport in L. casei ATCC 393 and L. plantarum 80. These two species could transport and metabolize D-xylose after transformation with plasmids which expressed the D-xylose-catabolizing genes of L. pentosus, xylAB. L. casei and L. plantarum mutants resistant to 2-deoxy-D-glucose were defective in EIIMan activity and were unable to transport D-xylose when transformed with plasmids containing the xylAB genes. Finally, transport of D-xylose was found to be the rate-limiting step in the growth of L. pentosus and of L. plantarum and L. casei ATCC 393 containing plasmids coding for the D-xylose-catabolic enzymes, since the doubling time of these bacteria on D-xylose was proportional to the level of EIIMan activity.

* Corresponding author. Mailing address: Department of Molecular Genetics and Gene Technology, TNO Nutrition and Food Research Institute, P.O. Box 360, 3700 AJ Zeist, The Netherlands. Phone: 31 30 6944 462. Fax: 31 30 6944 466. E-mail: Pouwels{at}voeding.tno.nl.

Journal of Bacteriology, August 1999, p. 4768-4773, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kawaguchi, H., Vertes, A. A., Okino, S., Inui, M., Yukawa, H. (2006). Engineering of a Xylose Metabolic Pathway in Corynebacterium glutamicum.. Appl. Environ. Microbiol. 72: 3418-3428 [Abstract] [Full Text]  
  • Abranches, J., Chen, Y.-Y. M., Burne, R. A. (2003). Characterization of Streptococcus mutans Strains Deficient in EIIABMan of the Sugar Phosphotransferase System. Appl. Environ. Microbiol. 69: 4760-4769 [Abstract] [Full Text]  
  • Acedo-Felix, E., Perez-Martinez, G. (2003). Significant differences between Lactobacillus casei subsp. casei ATCC 393T and a commonly used plasmid-cured derivative revealed by a polyphasic study. Int. J. Syst. Evol. Microbiol. 53: 67-75 [Abstract] [Full Text]  
  • Posthuma, C. C., Bader, R., Engelmann, R., Postma, P. W., Hengstenberg, W., Pouwels, P. H. (2002). Expression of the Xylulose 5-Phosphate Phosphoketolase Gene, xpkA, from Lactobacillus pentosus MD363 Is Induced by Sugars That Are Fermented via the Phosphoketolase Pathway and Is Repressed by Glucose Mediated by CcpA and the Mannose Phosphoenolpyruvate Phosphotransferase System. Appl. Environ. Microbiol. 68: 831-837 [Abstract] [Full Text]  
  • Chaillou, S., Postma, P. W., Pouwels, P. H. (2001). Contribution of the phosphoenolpyruvate:mannose phosphotransferase system to carbon catabolite repression in Lactobacillus pentosus. Microbiology 147: 671-679 [Abstract] [Full Text]  
  • Chervaux, C., Ehrlich, S. D., Maguin, E. (2000). Physiological Study of Lactobacillus delbrueckii subsp. bulgaricus Strains in a Novel Chemically Defined Medium. Appl. Environ. Microbiol. 66: 5306-5311 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»