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Journal of Bacteriology, August 1999, p. 5099-5102, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Disruption of narG, the Gene Encoding the Catalytic Subunit of Respiratory Nitrate Reductase, Also Affects Nitrite Respiration in Pseudomonas fluorescens YT101

Jean-François Ghiglione,1 Laurent Philippot,2 Philippe Normand,1 Robert Lensi,1 and Patrick Potier1,*

Laboratoire d'Ecologie Microbienne du Sol, UMR C.N.R.S. 5557, Université Claude Bernard, Lyon 1, 69622 Villeurbanne Cedex,1 and Laboratoire de Microbiologie des Sols, INRA-CMSE, B.V. 1540, 21034 Dijon Cedex,2 France

The Pseudomonas fluorescens YT101 gene narG, which encodes the catalytic alpha  subunit of the respiratory nitrate reductase, was disrupted by insertion of a gentamicin resistance cassette. In the Nar- mutants, nitrate reductase activity was not detectable under all the conditions tested, suggesting that P. fluorescens YT101 contains only one membrane-bound nitrate reductase and no periplasmic nitrate reductase. Whereas N2O respiration was not affected, anaerobic growth with NO2 as the sole electron acceptor was delayed for all of the Nar- mutants following a transfer from oxic to anoxic conditions. These results provide the first demonstration of a regulatory link between nitrate and nitrite respiration in the denitrifying pathway.

* Corresponding author. Mailing address: Laboratoire d'Ecologie Microbienne du Sol, UMR C.N.R.S. 5557, Université Claude Bernard, Lyon 1, 43 bd. du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. Phone: 33 4 72 43 13 78. Fax: 33 4 72 43 12 23. E-mail: potier{at}biomserv.univ-lyon1.fr.

Journal of Bacteriology, August 1999, p. 5099-5102, Vol. 181, No. 16
0021-9193/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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